A water-soluble and sensitive fluorescent probe N,N′-bis[tris-(2-aminoethyl)amine]-3,4,9,10-perylenetetracarboxylic diimide (PTRIS) was synthesized and, in combination with polydopamine (PDA), utilised in the detection of acetylcholinesterase (AChE) activity. PDA is spontaneously polymerized from dopamine (DA) in aerobic and alkaline solutions. The excellent absorption of PDA results in the aggregation of PTRIS around PDA as well as π–π stacking between them, which consequently quenched the fluorescence of PTRIS due to aggregation induced quenching (AIQ) in 9 min. The hydrolysis product of acetylthiocholine (ATCh) catalyzed by AChE, thiocholine (TCh), was proved to inhibit the polymerization of DA, therefore the free monomeric PTRIS retained its strong fluorescence intensity. The fluorescence was switched off and on depending on the activity of AChE. According to the change of fluorescence intensity at 550 nm, the detection limit of AChE was quantified as 0.02 mU mL⁻¹. It was also proved that this probe possessed excellent selectivity for AChE. Tacrine and the organophosphate pesticide diazinon were further evaluated for inhibitor screening. The half-maximal inhibitory concentration value of tacrine and diazinon was calculated to be 1.4 and 1.6 μM respectively, revealing potential applications for inhibition and pesticide detecting.

一种水溶性灵敏的荧光探针N , N'-双[三-（2-氨基乙基）胺]-3,4,9,10-苝四甲二亚胺（PTRIS）被合成，并与聚多巴胺（PDA）结合用于检测乙酰胆碱酯酶（AChE）活性。PDA 由多巴胺 (DA) 在有氧和碱性溶液中自发聚合而成。PDA 的优异吸收导致 PTRIS 在 PDA 周围聚集以及它们之间的 π-π 堆积，从而在 9 分钟内淬灭 PTRIS 的荧光，因为聚集诱导淬灭（AIQ）。乙酰硫代胆碱（ATCh）的水解产物硫代胆碱（TCh）被证明可以抑制 DA 的聚合，因此游离单体 PTRIS 保持了其强烈的荧光强度。根据 AChE 的活性关闭和打开荧光。^-1。还证明该探针对 AChE 具有出色的选择性。他克林和有机磷农药二嗪农进一步评估抑制剂筛选。他克林和二嗪农的半数抑制浓度值分别计算为 1.4 和 1.6 μM，揭示了其在抑制和农药检测方面的潜在应用。