The aims of the present study were to isolate and identify native entomopathogenic fungi, Beauveria bassiana from the Egyptian soil and to evaluate the artificial establishment of B. bassiana as endophytes in rice plants through seeds immersion. Ten soil samples were collected from different cultivated fields at the Ismailia Governorate. Only one sample was positive with a ratio of 10%. Sequencing of the internal transcribed spacer (ITS) region indicated that the isolate obtained from the soil sample belongs to B. bassiana and was registered under the accession no. MN337282. To test the endophytic colonization of B. bassiana, rice seeds were soaked by B. bassiana with a concentration of 5 × 107 spores/ml, to test when B. bassiana become an endophyte in rice plants. The plants were examined for endophytic presence of B. bassiana, 30 days post treatment. PCR amplification using fungal specific primers for a conserved region of β-tubulin gene yielded identical 360 bp products from both B. bassiana and rice treated plants. The results showed that seeds immersion with a conidial suspension proved to be a good method to introduce B. bassiana into rice leaves to protect the rice plants against stem borers.

中文翻译：

---

接种和分离昆虫病原真菌的定植白僵菌在水稻植物，水稻L.通过种子浸渍法

本研究的目的是从埃及土壤中分离和鉴定原生昆虫病原真菌 Beauveria bassiana，并评估通过种子浸泡在水稻植物中人工建立的球孢白僵菌作为内生菌。从伊斯梅利亚省的不同耕地收集了十个土壤样品。只有一个样本呈阳性，比例为 10%。内部转录间隔区 (ITS) 区域的测序表明，从土壤样品中获得的分离物属于 B. bassiana 并在登记号下注册。MN337282。为了测试球孢菌的内生定植，将水稻种子用球孢菌以 5 × 107 个孢子/ml 的浓度浸泡，以测试球孢菌何时成为水稻植株的内生菌。检查植物内生 B. bassiana，治疗后30天。使用真菌特异性引物对 β-微管蛋白基因的保守区域进行 PCR 扩增，从 B. bassiana 和水稻处理的植物中产生了相同的 360 bp 产物。结果表明，用分生孢子悬浮液浸泡种子被证明是一种将球孢菌引入水稻叶片以保护水稻植物免受螟虫侵害的好方法。