Pretreatment is a critical step required for efficient conversion of woody biomass into biofuels and platform chemicals. Fungal pretreatment is regarded as one of the most promising technology for woody biomass conversion but remains challenging for industrial application. The exploration of potential fungus strain with high efficient delignification and less processing time for woody biomass pretreatment will be valuable for development of biorefinery industry. Here, a newly isolated white-rot basidiomycete Peniophora incarnate T-7 was employed for poplar wood pretreatment. The chemical component analysis showed that cellulose, hemicellulose and lignin from poplar wood declined by 16%, 48% and 70%, respectively, after 7 days submerged fermentation by P. incarnate T-7. Enzymatic saccharification analysis revealed that the maximum yields of glucose and xylose from 7 days of P. incarnate T-7 treated poplar wood reached 33.4% and 27.6%, respectively, both of which were enhanced by sevenfold relative to the untreated group. Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), X-ray diffraction (XRD) and pyrolysis gas chromatography–mass spectrometry (Py-GC/MS) characterization confirmed that lignocellulosic structure of poplar wood was largely broken by P. incarnate T-7, including delignification and de-crystalline of cellulose. Meanwhile, lignin component of poplar wood was selectively degraded by P. incarnate T-7, and G-type unit of lignin was preferentially attacked by the strain. Furthermore, quantitative proteomic analysis revealed that a considerable amount of lignocellulolytic enzymes were detected in the secretory proteins of P. incarnate T-7, especially with high abundance of lignin-degrading enzymes and hemicellulases. Combination of quantitative proteomic with transcriptomic analysis results showed that most of those lignocellulolytic enzymes were highly upregulated on poplar wood substrate compared to glucose substrate. This study showed that P. incarnate T-7 could selectively delignify poplar wood by submerged fermentation with short time of 7 days, which greatly improved its enzymatic saccharification efficiency. Our results suggested that P. incarnate T-7 might be a promising candidate for industrial woody biomass pretreatment.

中文翻译：

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用新分离的白腐担子菌Peniophora incarnata T-7 通过深层发酵增强糖化作用对杨木进行选择性脱木素

预处理是将木质生物质有效转化为生物燃料和平台化学品所需的关键步骤。真菌预处理被认为是木质生物质转化最有前途的技术之一，但在工业应用中仍然具有挑战性。探索具有高效脱木素和较短处理时间的木质生物质预处理潜在真菌菌株，对于生物炼制工业的发展具有重要意义。在这里，一种新分离的白腐担子菌 Peniophora incarnate T-7 用于杨木木材预处理。化学成分分析表明，白杨木的纤维素、半纤维素和木质素在经过 P. incarnate T-7 浸没发酵 7 天后分别下降了 16%、48% 和 70%。酶促糖化分析表明，P. incarnate T-7 处理杨木 7 天后葡萄糖和木糖的最大产量分别达到 33.4% 和 27.6%，两者均比未处理组提高了 7 倍。傅里叶变换红外光谱 (FTIR)、扫描电子显微镜 (SEM)、X 射线衍射 (XRD) 和热解气相色谱-质谱 (Py-GC/MS) 表征证实杨木的木质纤维素结构在很大程度上被 P.化身 T-7，包括纤维素的脱木素和去结晶。同时，杨木的木质素组分被P. incarnate T-7选择性降解，并且该菌株优先攻击木质素的G型单元。此外，定量蛋白质组学分析表明，在 P. incarnate T-7 的分泌蛋白中检测到大量的木质纤维素分解酶，尤其是木质素降解酶和半纤维素酶的丰度较高。定量蛋白质组学与转录组学分析结果的结合表明，与葡萄糖底物相比，大多数这些木质纤维素分解酶在杨木底物上高度上调。本研究表明，P. incarnate T-7 可以在 7 天的短时间内通过深层发酵选择性地对杨木进行脱木素，大大提高了其酶促糖化效率。我们的结果表明 P. incarnate T-7 可能是工业木质生物质预处理的有希望的候选者。特别是含有大量木质素降解酶和半纤维素酶。定量蛋白质组学与转录组学分析结果的结合表明，与葡萄糖底物相比，大多数这些木质纤维素分解酶在杨木底物上高度上调。本研究表明，P. incarnate T-7 可以在 7 天的短时间内通过深层发酵选择性地对杨木进行脱木素，大大提高了其酶促糖化效率。我们的结果表明 P. incarnate T-7 可能是工业木质生物质预处理的有希望的候选者。特别是含有大量木质素降解酶和半纤维素酶。定量蛋白质组学与转录组学分析结果的结合表明，与葡萄糖底物相比，大多数这些木质纤维素分解酶在杨木底物上高度上调。本研究表明，P. incarnate T-7可以在7天的短时间内通过深层发酵选择性地对杨木进行脱木素，大大提高了其酶糖化效率。我们的结果表明 P. incarnate T-7 可能是工业木质生物质预处理的有希望的候选者。本研究表明，P. incarnate T-7 可以在 7 天的短时间内通过深层发酵选择性地对杨木进行脱木素，大大提高了其酶促糖化效率。我们的结果表明 P. incarnate T-7 可能是工业木质生物质预处理的有希望的候选者。本研究表明，P. incarnate T-7 可以在 7 天的短时间内通过深层发酵选择性地对杨木进行脱木素，大大提高了其酶促糖化效率。我们的结果表明 P. incarnate T-7 可能是工业木质生物质预处理的有希望的候选者。