Here the authors describe the development of AUTOptosis, an economical and rapid apoptosis monitoring method suitable for high-content and high-throughput screening assays. AUTOptosis is based on the quantification of nuclei intensity via staining with Hoechst 33342. First, the authors calibrated the method using standard apoptosis inducers in multiple cell lines. Next, the authors validated the applicability of this approach to high-content screening using a small library of compounds and compared it with the terminal deoxynucleotidyl transferase dUTP nick end labeling gold standard. Finally, the authors demonstrated the specificity of the method by using AUTOposis to detect apoptosis triggered by Mycobacterium tuberculosis intracellular infections.

中文翻译：

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高内涵和高通量筛选试验中的细胞凋亡评估。

在此，作者描述了 AUTOptosis 的发展，这是一种经济且快速的细胞凋亡监测方法，适用于高内涵和高通量筛选分析。AUTOptosis 基于通过使用 Hoechst 33342 染色对细胞核强度进行量化。首先，作者在多个细胞系中使用标准细胞凋亡诱导剂校准该方法。接下来，作者使用小型化合物库验证了这种方法在高内涵筛选中的适用性，并将其与末端脱氧核苷酸转移酶 dUTP 缺口末端标记金标准进行了比较。最后，作者通过使用 AUTOposis 检测由结核分枝杆菌细胞内感染引发的细胞凋亡证明了该方法的特异性。