Purpose

Cardiocephaloides comprises strigeid trematodes that represent a small genus. In this study, metacercaria identified as Cardiocephaloides sp. was collected from the Gangetic leaffish Nandus nandus from the Ganga River at Bairaj, Bijnor (29º01'N, 77º45'E) in the state of Uttar Pradesh (U.P.), India. Partial DNA sequences of the internal transcribed spacers (ITS1-5.8S-ITS2) and 28S gene of nuclear ribosomal DNA were generated and compared with available sequences of Cardiocephaloides species from Genbank database.

Methods

Encysted metacercariae of Cardiocephaloides sp. were collected from Nandus nandus were processed, identified and documented using morphological methods. The ITS1-5.8S-ITS2 cluster and 28S gene of ribosomal DNA of metacercariae were also sequenced and used for phylogenetic analysis.

Results

The infections of brain parasites are poorly understood in India and if studies are available, they are not properly described. During this study, the species collected were found belongs to the genus Cardiocephaloides. Metacercariae of Cardiocephaloides sp. is distinguished morphologically from others that also harbor brain by the presence of having an egg shape cyst and body elongate oval in shape with well-developed anterior part. The metacercariae are identified by matching of molecular sequence data and is compared to other species of Strigeidae.

Conclusion

This is the first record of metacercaria of Cardiocephaloides sp. from India. This molecular data from the present study will provide future comparative insights into species of Cardiocephaloides and its close affiliation to other congeners from different geographical areas.

中文翻译：

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Cardiocephaloides sp. Metacercaria 的描述。（Digenea，Diplostomoidea），从恒河叶鱼（Nandus nandus）的脑中新记录的及其在印度的遗传特征

目的

Cardiocephaloides包括strigeid吸虫表示一小属。在这项研究中，囊蚴被鉴定为Cardiocephaloides sp。从位于印度北方邦 (UP) 的比诺 (Bairaj, Bijnor (29º01'N, 77º45'E) 的恒河的恒河叶鱼 Nandus nandus中收集。生成内部转录间隔区 (ITS1-5.8S-ITS2) 的部分 DNA 序列和核核糖体 DNA 的 28S 基因，并与Genbank 数据库中的Cardiocephaloides物种的可用序列进行比较。

方法

的Encysted囊蚴Cardiocephaloides SP。使用形态学方法处理、鉴定和记录从南多斯中收集的南多斯。还对囊蚴核糖体DNA的ITS1-5.8S-ITS2簇和28S基因进行了测序并用于系统发育分析。

结果

在印度，人们对脑寄生虫感染知之甚少，即使有研究，也没有正确描述。在这项研究中，发现收集的物种属于Cardiocephaloides属。囊蚴Cardiocephaloides SP。在形态学上与其他也有大脑的人不同，它有一个蛋形囊肿和身体细长的椭圆形，前部发育良好。囊蚴是通过匹配分子序列数据来鉴定的，并与其他 Stigeidae 物种进行比较。

结论

这是Cardiocephaloides sp.囊蚴的第一个记录。来自印度。本研究的这一分子数据将提供未来对Cardiocephaloides物种及其与来自不同地理区域的其他同源物的密切联系的比较见解。