Abstract

This study has contributed in the description of bioaugmentation-phytoremediation efficiency process using Typha angustifolia concerning PCP tolerance and removal from wastewater. Samples of wastewater were collected from industrial wastewater plants, namely row wastewater effluent “E.WW”, primary wastewater “P.WW”, secondary wastewater “S.WW”, clarified wastewater “AC.WW”. These effluents were spiked with PCP at different rate (100, 500, and 1000 mg.L⁻¹), physical, chemical and biological properties were monitored. A second experiment was set up in order to check the efficiency of phytoremediation treatments of the different effluents artificially contaminated with 200 mg.L⁻¹ PCP after 20 days lab scale experiment. An important PCP removal by indigenous bacteria was showed in S. WW with values from 1000 to 72.2 mg.L⁻¹ from T0 (start of the experiment) to TF (end of the experiment), respectively. Phytoremediation process allowed a decrease of PCP rate from 200 to 6.4 mg.L⁻¹, a decrease of chloride content from 14.0 to 4.0 mg.L⁻¹ in S.WW samples was observed. Furthermore, a significant increase of bacterial number in S.WW and AC.WW to 1.700 × 10⁶ and 1.450 × 10⁶ CFU.mL⁻¹, respectively was observed. In addition, the DGGE analysis showed that after bioaugmentation-phytoremediation treatments, the highest species richness and relative abundance in wastewater effluent was observed.

Novelty statement Pentachlorophenol (PCP) is one of highly toxic of polychlorophenols and required to continuously monitor in environment. This paper presenting a sensitive method phytoremediation and bioaugmentation for PCP biotransformation in wastewater. The novelty is the choice of a macrophyte Typha angustifolia, which is still used for the elimination of heavy metals but it not used for pesticide and pollutant removal in wastewater. Also, there are different analysis that was performed in order to check phyto-technique process (DGGE and HPLC). On the other side, in this study, the phyto-techniques with Typha angustifolia positively affected intrinsic microorganisms in order to promote pollutant remediation. So, the intrinsic microorganisms in wastewater with the macrophyte presence have a great capacity to reduce this pollutant and improve the bioremediation process.

摘要

本研究有助于描述使用香蒲的生物强化-植物修复效率过程，涉及 PCP 耐受性和废水去除。从工业废水厂收集废水样品，即排废水“E.WW”、初级废水“P.WW”、二级废水“S.WW”、澄清废水“AC.WW”。这些流出物以不同的比率（100、500 和 1000 mg.L ^-1）添加五氯苯酚，监测物理、化学和生物特性。建立了第二个实验，以检查被 200 mg.L ^{-1人工污染的不同废水的植物修复处理效率。}20 天实验室规模实验后的 PCP。从 T0（实验开始）到 TF（实验结束），在S.WW 中显示了一种重要的 PCP 去除率，其值分别为 1000 至 72.2 mg.L ^{-1 。}植物修复过程允许 PCP 率从 200 降低到 6.4 mg.L ^-1，观察到 S.WW 样品中氯化物含量从 14.0 降低到 4.0 mg.L ^-1。此外，S.WW 和 AC.WW 中的细菌数量显着增加至 1.700 × 10 ⁶和 1.450 × 10 ⁶ CFU.mL ^-1, 分别观察到。此外，DGGE 分析表明，经过生物强化-植物修复处理后，废水中的物种丰富度和相对丰度最高。

新颖性声明五氯苯酚 (PCP) 是一种剧毒的多氯苯酚，需要在环境中持续监测。本文介绍了一种敏感的植物修复和生物强化方法，用于废水中的 PCP 生物转化。新颖之处在于选择了一种大型植物香蒲，它仍然用于去除重金属，但不用于去除废水中的农药和污染物。此外，为了检查植物技术过程（DGGE 和 HPLC），还进行了不同的分析。另一方面，在本研究中，香蒲的植物技术积极影响内在微生物，以促进污染物修复。因此，含有大型植物的废水中的固有微生物具有很强的减少这种污染物和改善生物修复过程的能力。