当前位置: X-MOL 学术Biotechnol. Biotechnol. Equip. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Molecular characterization and function analysis of the rice OsDUF617 family
Biotechnology & Biotechnological Equipment ( IF 1.5 ) Pub Date : 2021-06-12 , DOI: 10.1080/13102818.2021.1934541
Chunliu Li 1 , Dejia Hou 2 , Lin Zhang 1 , Xiaohong Li 1 , Jiangbo Fan 1 , Yilun Dong 1 , Jianqing Zhu 1 , Zhengjian Huang 1 , Zhengjun Xu 1, 3 , Lihua Li 1, 3
Affiliation  

Abstract

With the advance of sequencing technology, the number of sequenced genomes has been rapidly increasing. However, the functions of a large number of genes, including gene families with domains of unknown functions (DUF), still remain unclear. In this study, we analysed eight members of OsDUF617 (OsDUF617.1-OsDUF617.8) in rice Nipponbare. By phylogenetic analysis, all these OsDUF617 proteins could be classified into three major groups (I, II, III). We used real-time polymerase chain reaction to examine the expression of all these OsDUF617 members in 15 distinct rice tissues. The expression of these members under various abiotic stress and abscisic acid (ABA) conditions was also examined. Under drought conditions, the expression level of OsDUF617.8 in rice was significantly elevated. Overexpression of OsDUF617.8 in Escherichia coli led to a significant enhancement of catalase (CAT) and peroxidase (POD) activities, and improved bacterial resistance to osmotic stress. The results of this study will provide important information for further study of the function of the OsDUF617 family in rice.

Supplemental data for this article is available online at 10.1080/13102818.2021.1934541.



中文翻译:

水稻OsDUF617家族的分子特征及功能分析

摘要

随着测序技术的进步,已测序的基因组数量迅速增加。然而,大量基因的功能,包括具有未知功能域(DUF)的基因家族,仍不清楚。在本研究中,我们分析了日本晴水稻中 OsDUF617 的 8 个成员(OsDUF617.1-OsDUF617.8)。通过系统发育分析,所有这些 OsDUF617 蛋白都可以分为三大类(I、II、III)。我们使用实时聚合酶链反应来检查所有这些OsDUF617成员在 15 个不同水稻组织中的表达。还检查了这些成员在各种非生物胁迫和脱落酸 (ABA) 条件下的表达。干旱条件下,OsDUF617.8的表达水平在水稻中显着升高。的过表达OsDUF617.8大肠杆菌导致过氧化氢酶(CAT)和过氧化物酶(POD)活性的显著增强,和改进的渗透胁迫细菌耐药性。本研究结果将为进一步研究OsDUF617家族在水稻中的功能提供重要信息。

本文的补充数据可在 10.1080/13102818.2021.1934541 在线获得。

更新日期:2021-06-13
down
wechat
bug