Numerous byproducts resulting from chlorinated disinfection are constantly being generated during water treatment processes. The potential risks of these new emerging pollutions remain largely unknown. Here, we determined the risks of chlorinated disinfection byproducts of diazepam (DZP) in the cellular and zebrafish exposure experiments. The cytotoxicity of disinfection byproducts (MACB and MBCC) was greater than DZP in macrophage raw 264.7 cells at 10 mg/L. We further found that the effects of MBCC on the metabolism of glycine, serine, threonine and riboflavin were far greater than DZP by the targeted metabolomics methods. Moreover, MBCC significantly decreased the peak amplitude of neuronal action potential in primary embryonic rat (Spragu-Dawley SD) hippocampal neurons. We finally determined behavioral toxicity of DZP and byproducts in zebrafish larvae. MBCC significantly decreased the maximal swim-activity and peak duration of zebrafish after 72 h exposure. Altogether, these findings indicate the MBCC pose serious pressures on public health.

在水处理过程中不断产生氯化消毒产生的大量副产品。这些新出现的污染的潜在风险在很大程度上仍然未知。在这里，我们确定了细胞和斑马鱼暴露实验中地西泮 (DZP) 氯化消毒副产物的风险。在 10 mg/L 的巨噬细胞原始 264.7 细胞中，消毒副产物（MACB 和 MBCC）的细胞毒性大于 DZP。我们进一步发现，通过靶向代谢组学方法，MBCC对甘氨酸、丝氨酸、苏氨酸和核黄素代谢的影响远大于DZP。此外，MBCC 显着降低了原代胚胎大鼠 (Spragu-Dawley SD) 海马神经元中神经元动作电位的峰值幅度。我们最终确定了斑马鱼幼虫中 DZP 和副产品的行为毒性。MBCC 显着降低斑马鱼在暴露 72 小时后的最大游泳活动和峰值持续时间。总之，这些发现表明 MBCC 对公共卫生构成了严重压力。