Comprehensive analysis of DOK family genes expression, immune characteristics, and drug sensitivity in human tumors,Journal of Advanced Research

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Comprehensive analysis of DOK family genes expression, immune characteristics, and drug sensitivity in human tumors
Journal of Advanced Research ( IF 11.4 ) Pub Date : 2021-06-12 , DOI: 10.1016/j.jare.2021.06.008
Yongjun Guan ₁ , Man Li ₁ , Zhendong Qiu ₁ , Junhui Xu ₁ , Yichao Zhang ₁ , Ning Hu ₁ , Xuanzhe Zhang ₁ , Wenyi Guo ₁ , Jingping Yuan ₂ , Qiao Shi ₃ , Weixing Wang ₁

Affiliation

Introduction

DOK is a new type of regulatory protein family that participates in the regulation of tumor cell growth. However, most of the studies are conducted in cell lines, and systematic studies have not been conducted in human tumors.

Objectives

We conducted a comprehensive analysis of DOK based on its expression profile and its relationship with patient survival, immune infiltration, tumor microenvironment, and drug sensitivity.

Methods

We used the TCGA database to analyze the correlation between DOK family gene expression and prognosis and clinical stage. The protein expression of DOK in tumor tissues was analyzed by immunohistochemistry. Use the cBioPortal database to analyze the alteration frequency in DOK family genes in human tumors. In addition, we used ESTIMATE algorithm and TIMER website to analyze the correlation between DOK family genes and tumor immunity. Finally, we further analyzed the relationship between DOK family genes and tumor stemness and the sensitivity of cancer cells to chemotherapy.

Results

We conducted a comprehensive analysis of DOK family genes based on its expression profile and its relationship with patient survival. We also confirmed this conclusion by immunohistochemistry. The expression of DOK family genes is related to OS, clinical stage, tumor mutation, methylation, CNV, and SNV. DOK family genes are significantly associated with poor prognosis of UVM. DOK1-DOK3 has obvious correlation with tumor immunity. DOK2 can increase the sensitivity of chemotherapy drugs, while DOK4 reduces the sensitivity of multiple chemotherapy drugs. In addition, the expression level of DOK family genes is significantly correlated with the activity of cancer marker-related pathways.

Conclusions

DOK plays a role of tumor suppressor gene or tumor-promoting gene in different tumors. However, DOK family genes play a role in promoting cancer in UVM. DOK family genes are significantly associated with drug sensitivity.

中文翻译：

综合分析人类肿瘤中 DOK 家族基因的表达、免疫特征和药物敏感性

介绍

DOK是一种参与调控肿瘤细胞生长的新型调控蛋白家族。然而，大多数研究是在细胞系中进行的，尚未在人类肿瘤中进行系统研究。

目标

我们根据 DOK的表达谱及其与患者生存、免疫浸润、肿瘤微环境和药物敏感性的关系对DOK进行了综合分析。

方法

我们使用TCGA数据库分析了DOK家族基因表达与预后和临床分期的相关性。通过免疫组织化学分析DOK在肿瘤组织中的蛋白表达。使用 cBioPortal 数据库分析人类肿瘤中DOK家族基因的改变频率。此外，我们使用ESTIMATE算法和TIMER网站分析了DOK家族基因与肿瘤免疫的相关性。最后，我们进一步分析了DOK家族基因与肿瘤干性以及癌细胞对化疗敏感性的关系。

结果

我们根据其表达谱及其与患者生存的关系对DOK家族基因进行了全面分析。我们还通过免疫组织化学证实了这一结论。DOK家族基因的表达与OS、临床分期、肿瘤突变、甲基化、CNV和SNV有关。DOK家族基因与 UVM 的不良预后显着相关。DOK1-DOK3与肿瘤免疫有明显的相关性。DO K2可以增加化疗药物的敏感性，而DOK4可以降低多种化疗药物的敏感性。此外，DOK的表达水平家族基因与癌症标志物相关通路的活性显着相关。