Rho-dependent transcription termination regulates the toxin–antitoxin modules of cryptic prophages to silence their expression in Escherichia coli,FEBS Letters

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Rho-dependent transcription termination regulates the toxin–antitoxin modules of cryptic prophages to silence their expression in Escherichia coli
FEBS Letters ( IF 3.0 ) Pub Date : 2021-06-11 , DOI: 10.1002/1873-3468.14150
Mohammed Hafeezunnisa _{1,

2} , Passong Immanual R Chhakchhuak _{1,

3} , Janani Krishnakumar ₁ , Ranjan Sen ₁

Affiliation

Bacterial Rho-dependent transcription termination regulates many physiological processes. Here, we report that it controls the expression of toxin–antitoxin (TA) modules of cryptic prophages in E. coli. Microarray profiles of Rho mutants showed upregulation of genes of the CP4-6 and CP4-44 prophages, including their TA modules, that were validated by RT-qPCR. Analysis of the in vivo termination efficiency and the mRNA sequences of these prophages revealed the presence of many Rho-dependent terminators. The prophage TA modules exhibited synthetic lethality with the Rho mutants, indicating functional involvement of Rho-dependent termination in controlling these modules. Rho-dependent termination does not regulate most of the chromosomal TA modules. We conclude that Rho-dependent termination specifically silences the TA modules of prophages, thereby augmenting bacterial innate immunity.

中文翻译：

Rho 依赖性转录终止调节隐性原噬菌体的毒素-抗毒素模块以沉默它们在大肠杆菌中的表达

细菌 Rho 依赖性转录终止调节许多生理过程。在这里，我们报告说它控制大肠杆菌中神秘原噬菌体的毒素-抗毒素 (TA) 模块的表达。Rho 突变体的微阵列谱显示 CP4-6 和 CP4-44 原噬菌体的基因上调，包括它们的 TA 模块，通过 RT-qPCR 验证。体内分析这些原噬菌体的终止效率和 mRNA 序列揭示了许多 Rho 依赖性终止子的存在。原噬菌体 TA 模块对 Rho 突变体表现出合成致死性，表明 Rho 依赖性终止在控制这些模块中的功能参与。Rho 依赖性终止不调节大多数染色体 TA 模块。我们得出结论，Rho 依赖性终止特异性沉默原噬菌体的 TA 模块，从而增强细菌先天免疫。

更新日期：2021-08-09

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