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Phase-coded speckle illumination for laser Fourier ptychographic microscopy
Optics Communications ( IF 2.2 ) Pub Date : 2021-06-12 , DOI: 10.1016/j.optcom.2021.127199
Xiao Tao , Jinlei Zhang , Peng Sun , Chang Wang , Chenning Tao , Rengmao Wu , Zhenrong Zheng

It remains a tough task for designing a microscopy system to approach the diffraction limit over a wide field of view due to its restricted space bandwidth product (SBP). Fourier ptychographic microscopy (FPM) has been developed to broaden the SBP of a conventional microscope platform without major hardware modifications. In this paper, we propose a novel FPM system based on the phase-coded speckle illumination, termed PSI-FPM, which employs a laser source with a speckle illumination control optical system. A phase-only spatial light modulator (SLM) with a condenser lens is utilized in the PSI-FPM system to project the phase-coded speckle illumination patterns onto the sample. The convolution between the sample spectrum and the illumination spectrum in the Fourier domain extend the numerical aperture of the objective that acts as a low-pass filter with cut-off frequency. The SLM is introduced in the PSI-FPM system to produce the specific illumination patterns so that the number of the captured images used for reconstruction are greatly reduced. In addition, the generated pattern is scanned to different directions without mechanical movement, which improves the robustness of the system. The PSI-FPM system is validated against simulations and experiments, and as a result reconstructs high-resolution images once the phase retrieval algorithm is employed. We envision that the PSI-FPM system will be suitable for many other speckle imaging applications.



中文翻译:

用于激光傅里叶 ptychographic 显微镜的相位编码散斑照明

由于其有限的空间带宽积 (SBP),设计显微镜系统以在宽视场内接近衍射极限仍然是一项艰巨的任务。傅立叶 ptychographic 显微镜 (FPM) 已被开发用于扩展传统显微镜平台的 SBP,而无需对硬件进行重大修改。在本文中,我们提出了一种基于相位编码散斑照明的新型 FPM 系统,称为 PSI-FPM,它采用具有散斑照明控制光学系统的激光源。在 PSI-FPM 系统中,使用带有聚光透镜的仅相位空间光调制器 (SLM) 将相位编码散斑照明图案投影到样品上。傅里叶域中样本光谱和照明光谱之间的卷积扩展了物镜的数值孔径,该物镜充当具有截止频率的低通滤波器。PSI-FPM 系统中引入了 SLM 以产生特定的照明模式,从而大大减少了用于重建的捕获图像的数量。此外,生成的图案在没有机械运动的情况下被扫描到不同的方向,这提高了系统的鲁棒性。PSI-FPM 系统经过模拟和实验验证,因此一旦采用相位检索算法,就可以重建高分辨率图像。我们设想 PSI-FPM 系统将适用于许多其他散斑成像应用。PSI-FPM 系统中引入了 SLM 以产生特定的照明模式,从而大大减少了用于重建的捕获图像的数量。此外,生成的图案在没有机械运动的情况下被扫描到不同的方向,这提高了系统的鲁棒性。PSI-FPM 系统经过模拟和实验验证,因此一旦采用相位检索算法,就可以重建高分辨率图像。我们设想 PSI-FPM 系统将适用于许多其他散斑成像应用。PSI-FPM 系统中引入了 SLM 以产生特定的照明模式,从而大大减少了用于重建的捕获图像的数量。此外,生成的图案在没有机械运动的情况下被扫描到不同的方向,这提高了系统的鲁棒性。PSI-FPM 系统经过模拟和实验验证,因此一旦采用相位检索算法,就可以重建高分辨率图像。我们设想 PSI-FPM 系统将适用于许多其他散斑成像应用。PSI-FPM 系统经过模拟和实验验证,因此一旦采用相位检索算法,就可以重建高分辨率图像。我们设想 PSI-FPM 系统将适用于许多其他散斑成像应用。PSI-FPM 系统经过模拟和实验验证,因此一旦采用相位检索算法,就可以重建高分辨率图像。我们设想 PSI-FPM 系统将适用于许多其他散斑成像应用。

更新日期:2021-06-23
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