Obesity is associated with changes in amino acid metabolism, and studies show that ingestion of fish proteins influence amino acid composition in plasma and urine, in addition to affecting risk factors for metabolic syndrome. Since the majority of fish proteins consumed by humans are as fish fillet, it is of interest to investigate if cod fillet intake affects amino acid composition and metabolic disorders. We hypothesized that a modified AIN-93G diet containing cod fillet would affect amino acid compositions in plasma and urine in obese rats, and also affect risk factors for metabolic syndrome when compared to rats fed a regular AIN-93G diet with casein as the protein source. Obese Zucker fa/fa rats, a rat model of metabolic syndrome, received diets containing 25% protein from lyophilized baked cod fillet and 75% protein from casein (Baked cod diet), or a Control diet with casein for four weeks. The Baked cod diet affected the amino acid composition in plasma, with e.g., lower glycine, histidine, homoarginine, homocysteine, methionine, proline and tyrosine concentrations, but did not affect amino acid concentrations in urine. The concentrations of markers for kidney and liver dysfunction were lower in the Baked cod group, however blood pressure development, fasting and postprandial glucose, and hepatic triacylglycerol concentrations were similar to the Control group. To conclude, substituting 25% of dietary protein with baked cod fillet affected concentrations of some amino acids in plasma and delayed development of kidney and liver dysfunction, but did not affect blood pressure, glucose concentration or fatty liver.

肥胖与氨基酸代谢的变化有关，研究表明，摄入鱼蛋白除了影响代谢综合征的危险因素外，还会影响血浆和尿液中的氨基酸组成。由于人类消耗的大多数鱼蛋白都是鱼片，因此研究鳕鱼片的摄入是否会影响氨基酸组成和代谢紊乱是很有意义的。我们假设，与饲喂以酪蛋白作为蛋白质来源的常规 AIN-93G 饮食的大鼠相比，含有鳕鱼片的改良 AIN-93G 饮食会影响肥胖大鼠血浆和尿液中的氨基酸组成，并且还会影响代谢综合征的危险因素。肥胖 Zucker fa/fa ​​大鼠是一种代谢综合征大鼠模型，接受含 25% 冻干烤鳕鱼片蛋白质和 75% 酪蛋白蛋白质（烤鳕鱼饮食）的饮食，或含酪蛋白的对照饮食，为期 4 周。烤鳕鱼饮食影响血浆中的氨基酸组成，例如降低甘氨酸、组氨酸、高精氨酸、高半胱氨酸、蛋氨酸、脯氨酸和酪氨酸浓度，但不影响尿液中的氨基酸浓度。烤鳕鱼组的肾和肝功能障碍标志物浓度较低，但血压变化、空腹和餐后血糖以及肝三酰甘油浓度与对照组相似。总之，用烤鳕鱼片替代 25% 的膳食蛋白质会影响血浆中某些氨基酸的浓度，并延缓肾和肝功能障碍的发展，但不会影响血压、血糖浓度或脂肪肝。