Abstract

Eragrostis tef is a increasingly becoming popular in different parts of the world as cereal due to its wide acceptability in extreme environmental conditions where most other cereals failed to make impact. NF-X1-like proteins belongs to transcription factors and involved in variety of processes during the life time of plants. Only few reports are available for NF-X1-like proteins in different repository which makes the prediction of function and structure for NF-X1 genes from tef. However, in the current study with little literature and lack of characterized NF-X1 genes attempts has been made to fill the gaps in the required areas. In this study, 49 NFX-like sequences belonging to 17 different plants were compared with four NFX-like sequences from tef. On analysis it was found that tef NF-X1 genes have RING finger domains, cysteine rich residues in the conserved region and PHP domain like other plants which helps in modulation of the targets. The phylogenetic analysis depicted evolutionary significance of almost similar nature of these genes in monocots and dicots with tobacco and tomato emerging as the closest relatives. The promoter analysis revealed conserved cis-regulatory elements in analyzed species and points towards similar putative function in tef as well. The observations and analysis is suitable for understanding differential structural and functional aspects of NF-X1-like genes in Eragrostis tef and other plants.

中文翻译：

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用于揭示未充分利用的谷物画眉草 tef 中 NF-X1 样蛋白的结构和功能作用的计算机方法

摘要

画眉草由于其在大多数其他谷物无法产生影响的极端环境条件下的广泛可接受性，它在世界不同地区越来越受欢迎。NF-X1 样蛋白属于转录因子，参与植物生命周期的各种过程。对于不同存储库中的 NF-X1 样蛋白，只有很少的报告可用，这使得从 tef 中预测 NF-X1 基因的功能和结构。然而，在目前文献很少且缺乏特征性 NF-X1 基因的研究中，已尝试填补所需领域的空白。在这项研究中，属于 17 种不同植物的 49 个 NFX 样序列与来自 tef 的四个 NFX 样序列进行了比较。分析发现 tef NF-X1 基因具有无名指结构域，像其他植物一样，在保守区域和 PHP 结构域中富含半胱氨酸的残基有助于调节目标。系统发育分析描述了这些基因在单子叶植物和双子叶植物中几乎相似性质的进化意义，烟草和番茄作为最近的亲戚出现。启动子分析显示保守分析物种中的顺式调节元件，并指向 tef 中类似的推定功能。观察和分析适用于了解画眉草和其他植物中 NF-X1 样基因的差异结构和功能方面。