Members of Hymenochaetaceae fungi are among well-known macromycetes with various medicinal properties. The aim of this study was to investigate the biological activities of Phellinus tuberculosus and Fuscoporia ferruginosa collected in Iran. The antimicrobial, antioxidant, and cytotoxic activities of the two species were examined, and their phenolic and polysaccharide contents were quantified. Compounds were characterized by HPLC–DAD chromatography and LC–ESI–MS/MS spectroscopy. According to our results, the antibacterial and antioxidant effects of P. tuberculosus extracts were stronger than F. ferruginosa. Also, the effect of hydroalcoholic extracts was higher than the aqueous extract. Gram-positive bacteria were more sensitive to all extracts, especially Streptococcus mutans with a MIC of 0.7 mg/mL and MBC of 6.25 mg/mL. HPLC–DAD analyses detected gallic acid, caffeic acid, and syringic acid in both fungi. The LC–ESI–MS/MS confirmed the detected compounds in HPLC–DAD and showed the presence of several phenolic compounds such as phellifuropyranone, phelligridin, and hispidin, besides others. This study showed that F. ferruginosa and P. tuberculosus are potent medicinal fungi with antibacterial and antioxidant properties, with no toxic effect on normal HDF cells, and possess various bioactive compounds including styrylpyrone-type phenols with well-known bioactivities.

Hymenochaetaceae 真菌的成员是众所周知的具有各种药用特性的大型菌之一。本研究的目的是调查在伊朗采集的桑黄和铁锈菌的生物活性。检查了这两个物种的抗微生物、抗氧化和细胞毒活性，并量化了它们的酚类和多糖含量。化合物通过 HPLC-DAD 色谱和 LC-ESI-MS/MS 光谱表征。根据我们的研究结果，结核菌提取物的抗菌和抗氧化作用强于铁锈菌. 此外，水醇提取物的效果高于水提取物。革兰氏阳性菌对所有提取物更敏感，尤其是变形链球菌，MIC 为 0.7 mg/mL，MBC 为 6.25 mg/mL。HPLC-DAD 分析检测到两种真菌中的没食子酸、咖啡酸和丁香酸。LC-ESI-MS/MS 确认了 HPLC-DAD 中检测到的化合物，并显示除其他化合物外，还存在几种酚类化合物，如 phellifuropyranone、phelligridin 和 hispidin。该研究表明F. ferruginosa和P.tuberculosus 是有效的药用真菌，具有抗菌和抗氧化特性，对正常 HDF 细胞没有毒性作用，并具有多种生物活性化合物，包括具有众所周知的生物活性的苯乙烯吡喃酮型酚类。