Nuclear magnetic resonance (NMR) is one of two methods (in addition to MS) that predominate in metabolomics and structural analysis. Due to the great demand for this type of research, there is a very high consumption of solvents. Fortunately, most biological studies can be performed using simple protocols that include 10% D₂O and 90% H₂O. More complex metabolomics studies with polar compound extraction require the use of MeOH or MeCN as reagents for protein precipitation. The most extensive Folch extraction method requires a mixture of solvents (MeOH/H₂O/CHCl₃) for tissue metabolites and sometimes biofluid extraction. In this review, we show that NMR can be a green method for most biological studies that yields useful information where the overall approach is in line with the GSK (GlaxoSmithKline) solvent sustainability guide.

核磁共振 (NMR) 是在代谢组学和结构分析中占主导地位的两种方法之一（除 MS 外）。由于对此类研究的巨大需求，溶剂的消耗量非常高。幸运的是，大多数生物学研究可以使用包括 10% D ₂ O 和 90% H ₂ O 的简单方案进行。使用极性化合物提取的更复杂的代谢组学研究需要使用 MeOH 或 MeCN 作为蛋白质沉淀试剂。最广泛的 Folch 提取方法需要混合溶剂 (MeOH/H ₂ O/CHCl ₃) 用于组织代谢物，有时用于生物流体提取。在这篇综述中，我们表明 NMR 可以成为大多数生物学研究的绿色方法，可产生有用的信息，其中整体方法符合 GSK（葛兰素史克）溶剂可持续性指南。