ABSTRACT

Conventional antimicrobial therapies are less effective against pythiosis. Therefore, the goal of this study was to characterize the effects of two ozone (O₃) presentations on the growth of canine Pythium insidiosum. In Experiment 1, the pathogen was treated with O₃ gas at different concentrations (32, 52, or 72 μg O₃ mL⁻¹ for 30 min) or exposure times (72 μg O₃ mL⁻¹ for 5, 15, or 30 min). In Experiment 2, pathogen samples were covered with ozonized sunflower oil with distinct peroxide indices. The growth area of P. insidiosum was measured during the first 14 days after treatment. Samples without growth were recultured to assess the germicidal or germistatic action of the respective therapy. In Experiment 1, treatment for 30 min had germicidal action regardless of the gas concentration, whereas shorter exposures to O₃ were not effective. In Experiment 2, only ozonized sunflower oil with a peroxide index > 600 mmol equiv. kg⁻¹ inactivated the pathogen. The findings of the present study suggest that exposure to O₃ gas and ozonized sunflower oil with a high peroxide index are promising options for the long-lasting treatment of cutaneous pythiosis in humans and domestic animals.

摘要

传统的抗微生物疗法对脓毒症的效果较差。因此，本研究的目的是描述两种臭氧 (O ₃ ) 呈现对犬内腐霉生长的影响。在实验 1 中，用不同浓度（32、52 或 72 μg O ₃ mL ^-1 30 分钟）或暴露时间（72 μg O ₃ mL ^-1 5、15 或30 分钟）的 O ₃气体处理病原体分钟）。在实验 2 中，病原体样品覆盖有不同过氧化物指数的臭氧化葵花油。P. insidiosum的生长区域在治疗后的前 14 天测量。再培养没有生长的样品以评估相应疗法的杀菌或抑菌作用。在实验 1 中，无论气体浓度如何，处理 30 分钟都具有杀菌作用，而短时间暴露于 O ₃则无效。在实验 2 中，仅使用过氧化物指数 > 600 mmol equiv 的臭氧化葵花籽油。kg ^-1灭活病原体。本研究的结果表明，暴露于 O ₃气体和具有高过氧化物指数的臭氧化葵花油是长期治疗人类和家畜皮肤腐烂病的有希望的选择。