ABSTRACT

Jatropha mollissima is used in folk medicine as antimicrobial, antiparasitic, and larvicidal. However, few toxicogenetic studies have been carried out. Therefore, the aim of this study was to determine the phytochemical profile of ethanolic leaf extract of J. mollissima (EEJM) as well as potential cytotoxic, mutagenic, and antimutagenic properties. The EEJM was subjected to successive fractionation for the isolation of secondary metabolites, and five concentrations (0.01; 0.1; 1; 10 and 100 mg/ml) of extract were investigated using Allium cepa assay and the Somatic Mutation and Recombination (SMART) test. The mitotic index and % damage reduction were analyzed for A. cepa and the frequency of mutant hair for SMART. The presence of coumarins, alkaloids, flavonoids, saponins, and tannins was detected, while spinasterol and n-triacontane were the isolates identified for the first time for this species. EEJM did not exhibit cytotoxicity and was not mutagenic at 1 or 10 mg/ml using A. cepa and all concentrations of EEJM were not mutagenic in the SMART test. A cytoprotective effect was found at all concentrations. At 1 or 10 mg/ml EEJM exhibited antimutagenicity in A. cepa. In SMART, the protective effect was observed at 0.1 to 100 mg/ml EEJM. Our results demonstrate the important chemopreventive activity of EEJM, a desired quality in the search for natural anticarcinogenic compounds.

摘要

麻风树在民间医学中用作抗菌剂、抗寄生虫剂和杀幼虫剂。然而，很少进行毒理学研究。因此，本研究的目的是确定J. mollissima (EEJM)乙醇叶提取物的植物化学特征以及潜在的细胞毒性、诱变和抗诱变特性。对 EEJM 进行连续分级以分离次级代谢物，并使用洋葱分析法和体细胞突变和重组 (SMART) 测试研究了五种浓度（0.01；0.1；1；10 和 100 毫克/毫升）的提取物。分析了A. cepa的有丝分裂指数和损伤减少百分比以及 SMART 突变头发的频率。检测到香豆素、生物碱、黄酮类化合物、皂苷和单宁的存在，而菠菜甾醇和正三十烷是该物种首次鉴定的分离物。EEJM 没有表现出细胞毒性，并且在 1 或 10 mg/ml 下使用A. cepa没有致突变性，并且所有浓度的 EEJM 在 SMART 测试中都没有致突变性。在所有浓度下都发现了细胞保护作用。1 或 10 mg/ml EEJM 在A. cepa 中表现出抗突变性。在 SMART 中，在 0.1 至 100 mg/ml EEJM 下观察到保护作用。我们的结果证明了 EEJM 的重要化学预防活性，这是寻找天然抗癌化合物的理想品质。