Nurseries are crucial habitats that play an important role for many marine fish species; which rely on them to complete their life cycle. Juvenile stages of dab (Limanda limanda), plaice (Pleuronectes platessa) and sole (Solea solea) present in the English Channel are common in the Seine estuary nursery grounds. To further explore the estuary's nursery function, we investigated the heterogeneity in spatial distribution patterns of these three flatfish at their juvenile stage, between 1996 and 2019. We used geostatistical indices and multivariate analyses to demonstrate species specific spatiotemporal dynamic. Sole favoured the most upstream part of the nursery, dab was found in the most marine areas, and plaice preferred the southern coast. We then performed clusters analysis based on spatial indices and spatial patterns extracted from a Minimum/Maximum Autocorrelation Factor (MAF). We showed that the average positions of the three flatfish species were stable across time. Each flatfish appeared to have its own spatial preference inside the nursery. No temporal variability in the spatial pattern nor trend was found that would correspond to the major stress imposed on the community by harbour development within the 2000–2005 period. We conclude that segregation of the juveniles of the three species within the nursery may reflect different ecological needs and underlying mechanisms to minimise interspecific competition.

苗圃是重要的栖息地，对许多海洋鱼类物种发挥着重要作用；依靠它们来完成它们的生命周期。幼鱼 ( Limanda limanda )、鲽 ( Pleuronectes platesa ) 和鲽( Solea solea)) 存在于英吉利海峡，常见于塞纳河河口的苗圃。为了进一步探索河口的育苗功能，我们调查了这三种比目鱼在 1996 年至 2019 年期间幼年阶段空间分布模式的异质性。我们使用地统计指数和多变量分析来证明特定物种的时空动态。鲽喜欢苗圃最上游的部分，在大部分海域发现了轻吻鲽，而鲽喜欢南部海岸。然后，我们根据从最小/最大自相关因子 (MAF) 中提取的空间索引和空间模式进行聚类分析。我们表明三种比目鱼物种的平均位置随着时间的推移是稳定的。每条比目鱼在育苗场内似乎都有自己的空间偏好。没有发现与 2000-2005 年期间港口开发给社区带来的主要压力相对应的空间格局或趋势的时间变化。我们得出的结论是，苗圃内三个物种幼鱼的隔离可能反映了不同的生态需求和最小化种间竞争的潜在机制。