Quantification and mapping of DNA modifications,RSC Chemical Biology

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Quantification and mapping of DNA modifications
RSC Chemical Biology ( IF 4.2 ) Pub Date : 2021-5-21 , DOI: 10.1039/d1cb00022e
Yi Dai ₁ , Bi-Feng Yuan _{1,

2} , Yu-Qi Feng _{1,

2}

Affiliation

Apart from the four canonical nucleobases, DNA molecules carry a number of natural modifications. Substantial evidence shows that DNA modifications can regulate diverse biological processes. Dynamic and reversible modifications of DNA are critical for cell differentiation and development. Dysregulation of DNA modifications is closely related to many human diseases. The research of DNA modifications is a rapidly expanding area and has been significantly stimulated by the innovations of analytical methods. With the recent advances in methods and techniques, a series of new DNA modifications have been discovered in the genomes of prokaryotes and eukaryotes. Deciphering the biological roles of DNA modifications depends on the sensitive detection, accurate quantification, and genome-wide mapping of modifications in genomic DNA. This review provides an overview of the recent advances in analytical methods and techniques for both the quantification and genome-wide mapping of natural DNA modifications. We discuss the principles, advantages, and limitations of these developed methods. It is anticipated that new methods and techniques will resolve the current challenges in this burgeoning research field and expedite the elucidation of the functions of DNA modifications.

中文翻译：

DNA 修饰的定量和绘图

除了四种典型核碱基外，DNA 分子还带有许多天然修饰。大量证据表明 DNA 修饰可以调节多种生物过程。 DNA 的动态和可逆修饰对于细胞分化和发育至关重要。 DNA修饰失调与许多人类疾病密切相关。 DNA 修饰研究是一个迅速扩展的领域，分析方法的创新极大地促进了这一领域的发展。随着方法和技术的最新进展，在原核生物和真核生物的基因组中发现了一系列新的DNA修饰。破译 DNA 修饰的生物学作用取决于基因组 DNA 修饰的灵敏检测、准确定量和全基因组图谱。这篇综述概述了天然 DNA 修饰的定量和全基因组图谱分析方法和技术的最新进展。我们讨论这些开发方法的原理、优点和局限性。预计新的方法和技术将解决这一新兴研究领域当前的挑战，并加速阐明 DNA 修饰的功能。

更新日期：2021-06-14

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