The possibility of determining the active substances of antibacterial drugs of the tetracycline series (tetracycline, doxycycline, oxytetracycline, demeclocycline, metacycline, and chlortetracycline) using solid-phase fluorescence digital colorimetry is considered. The intrinsic fluorescence of tetracyclines and tetracycline-sensitized fluorescence of europium on cellulose paper and a thin layer of silica gel was studied. Yellow-green (tetracyclines) and pink (europium) fluorescence were observed upon irradiation with UV light (365 nm) of spots of tetracycline solutions applied to the matrix. The fluorescence intensity on the matrix surface was measured using a smartphone. Values of colorimetric parameters in the RGB system were used as the analytical signals (A_r). The limits of detection and quantitation were 1 – 5 and 3 – 17 μg/mL, respectively, for all analytes. The ranges of the determined concentrations were 3 – 500 μg/mL. The technique was tested for medicinal products. The relative standard deviation of the analytical results was ≤0.06.

考虑了使用固相荧光数字比色法测定四环素系列抗菌药物（四环素、强力霉素、土霉素、地美环素、美他环素和金霉素）的活性物质的可能性。研究了四环素的固有荧光和铕在纤维素纸和硅胶薄层上的四环素敏化荧光。在用紫外光 (365 nm) 照射施加到基质上的四环素溶液斑点后，观察到黄绿色（四环素）和粉红色（铕）荧光。使用智能手机测量基质表面的荧光强度。RGB 系统中的比色参数值被用作分析信号 ( A _r）。所有分析物的检测限和定量限分别为 1 – 5 和 3 – 17 µg/mL。测定的浓度范围为 3 – 500 μg/mL。该技术已在医药产品中进行了测试。分析结果的相对标准偏差≤0.06。