This article develops the reference measurement procedure (RMP), including acid hydrolysis, Randall extraction, evaporation of solvent, and weighing of dry residue in the determination of mass fraction of fat content. This paper’s RMP applies to dairy, meat, and egg products, and to baby food. In order to develop this RMP, selection and optimization of hydrolysis and extraction parameters were carried out. The paper takes into consideration the components of measurement uncertainty arising from the contributions of equipment and reagents. It also evaluates measurement equations and methodological factors. This article also presents the results of an inter-laboratory comparison of the determination of mass fraction of fat in Russian enterprises’ dairy products. These results demonstrate that the RMP developed by this research had the highest level of accuracy and the absence of bias in the measurement results as compared to other routine methods. Experiments carried out during the participation of UNIIM in an inter-laboratory comparison organized by MUVA Kempten GmbH (Germany) confirmed the equivalence of the RMP developed by this research with similar foreign measurement methods. The results of the work will ensure coherence of the results of determining the fat content in food products and food raw materials in the Russian Federation, and their conformity with the results obtained in other countries.

本文开发了参考测量程序 (RMP)，包括酸水解、Randall 萃取、溶剂蒸发和干残渣称量，用于测定脂肪含量的质量分数。本文的 RMP 适用于乳制品、肉类和蛋制品以及婴儿食品。为了开发该 RMP，进行了水解和提取参数的选择和优化。本文考虑了由设备和试剂的贡献引起的测量不确定度的组成部分。它还评估测量方程和方法因素。本文还介绍了对俄罗斯企业乳制品中脂肪质量分数测定的实验室间比较结果。这些结果表明，与其他常规方法相比，本研究开发的 RMP 具有最高水平的准确性和测量结果中没有偏差。在 UNIIM 参与由 MUVA Kempten GmbH（德国）组织的实验室间比较期间进行的实验证实了本研究开发的 RMP 与国外类似测量方法的等效性。这项工作的结果将确保俄罗斯联邦食品和食品原料中脂肪含量测定结果的一致性，以及它们与其他国家获得的结果的一致性。在 UNIIM 参与由 MUVA Kempten GmbH（德国）组织的实验室间比较期间进行的实验证实了本研究开发的 RMP 与国外类似测量方法的等效性。这项工作的结果将确保俄罗斯联邦食品和食品原料中脂肪含量测定结果的一致性，以及它们与其他国家获得的结果的一致性。在 UNIIM 参与由 MUVA Kempten GmbH（德国）组织的实验室间比较期间进行的实验证实了本研究开发的 RMP 与国外类似测量方法的等效性。这项工作的结果将确保俄罗斯联邦食品和食品原料中脂肪含量测定结果的一致性，以及它们与其他国家获得的结果的一致性。