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Unveiling the CoA mediated salicylate catabolic mechanism in Rhizobium sp. X9
Molecular Microbiology ( IF 3.6 ) Pub Date : 2021-06-14 , DOI: 10.1111/mmi.14771
Yidong Zhou 1 , Siyuan Gao 1 , Mingliang Zhang 1 , Wankui Jiang 1 , Zhijian Ke 1 , Jiguo Qiu 1 , Jianhong Xu 2 , Qing Hong 1
Affiliation  

Salicylate is a typical aromatic compound widely distributed in nature. Microbial degradation of salicylate has been well studied and salicylate hydroxylases play essential roles in linking the peripheral and ring-cleavage catabolic pathways. The direct hydroxylation of salicylate catalyzed by salicylate-1-hydroxylase or salicylate-5-hydroxylase has been well studied. However, the CoA mediated salicylate 5-hydroxylation pathway has not been characterized in detail. Here, we elucidate the molecular mechanism of the reaction in the conversion of salicylate to gentisate in the carbaryl-degrading strain Rhizobium sp. X9. Three enzymes (salicylyl-CoA ligase CehG, salicylyl-CoA hydroxylase CehH and gentisyl-CoA thioesterase CehI) catalyzed the conversion of salicylate to gentisate via a route, including CoA thioester formation, hydroxylation and thioester hydrolysis. Further analysis indicated that genes cehGHI are also distributed in other bacteria from terrestrial environment and marine sediments. These genomic evidences highlight the role of this salicylate degradation pathway in the carbon cycle of soil organic compounds and marine sediments. Our findings of this three-step strategy enhanced the current understanding of CoA mediated degradation of salicylate.

中文翻译:

揭示根瘤菌中 CoA 介导的水杨酸分解代谢机制。X9

水杨酸盐是一种在自然界广泛分布的典型芳香族化合物。水杨酸盐的微生物降解已得到充分研究,水杨酸盐羟化酶在连接外周和环切割分解代谢途径中起重要作用。由水杨酸-1-羟化酶或水杨酸-5-羟化酶催化的水杨酸直接羟基化已得到充分研究。然而,尚未详细表征 CoA 介导的水杨酸 5-羟基化途径。在这里,我们阐明了甲萘威降解菌株根瘤菌中水杨酸盐转化为龙胆酸盐的反应的分子机制。sp。X9。三种酶(水杨酰辅酶A连接酶CehG、水杨酰辅酶A羟化酶CehH和龙胆酰辅酶A硫酯酶CehI)通过包括辅酶A硫酯形成、羟基化和硫酯水解在内的途径催化水杨酸转化为龙胆酸。进一步的分析表明,cehGHI基因也分布在来自陆地环境和海洋沉积物的其他细菌中。这些基因组证据强调了这种水杨酸盐降解途径在土壤有机化合物和海洋沉积物的碳循环中的作用。我们对这一三步策略的发现增强了目前对 CoA 介导的水杨酸盐降解的理解。
更新日期:2021-06-14
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