The aim of this study was to investigate the potential of novel electrospun fiber mats loaded with alkannin and shikonin (A/S) derivatives, using as carrier a highly biocompatible, bio-derived, eco-friendly polymer such as poly[(R)-3-hydroxybutyric acid] (PHB). PHB fibers containing a mixture of A/S derivatives at different ratios were successfully fabricated via electrospinning. Αs evidenced by scanning electron microscopy, the fibers formed a bead-free mesh with average diameters from 1.25 to 1.47 μm. Spectroscopic measurements suggest that electrospinning marginally increases the amorphous content of the predominantly crystalline PHB in the fibers, while a significant drug amount lies near the fiber surface for samples of high total A/S content. All scaffolds displayed satisfactory characteristics, with the lower concentrations of A/S mixture-loaded PHB fiber mats achieving higher porosity, water uptake ratios, and entrapment efficiencies. The in vitro dissolution studies revealed that all samples released more than 70% of the encapsulated drug after 72 h. All PHB scaffolds tested by cell viability assay were proven non-toxic for Hs27 fibroblasts, with the 0.15 wt.% sample favoring cell attachment, spreading onto the scaffold surface, as well as cell proliferation. Finally, the antimicrobial activity of PHB meshes loaded with A/S mixture was documented for Staphylococcus epidermidis and S. aureus.

中文翻译：

---

新型电纺聚羟基丁酸酯支架作为伤口愈合剂阿卡宁和紫草素的载体


本研究的目的是研究负载阿尔坎宁和紫草素 (A/S) 衍生物的新型静电纺纤维垫的潜力，使用高度生物相容性、生物衍生的、环境友好的聚合物（如聚[(R)- 3-羟基丁酸](PHB)。通过静电纺丝成功制备了含有不同比例的 A/S 衍生物混合物的 PHB 纤维。扫描电子显微镜证明，纤维形成平均直径为1.25至1.47μm的无珠网。光谱测量表明，静电纺丝略微增加了纤维中主要结晶 PHB 的无定形含量，而对于总 A/S 含量较高的样品，显着的药物量位于纤维表面附近。所有支架均表现出令人满意的特性，较低浓度的 A/S 混合物负载的 PHB 纤维垫实现了较高的孔隙率、吸水率和截留效率。体外溶出研究表明，72 小时后，所有样品释放了超过 70% 的包封药物。通过细胞活力测定测试的所有 PHB 支架均被证明对 Hs27 成纤维细胞无毒，0.15 wt.% 的样品有利于细胞附着、扩散到支架表面以及细胞增殖。最后，记录了负载 A/S 混合物的 PHB 网片对表皮葡萄球菌和金黄色葡萄球菌的抗菌活性。