Abstract

Zinc oxide nanoparticles are incorporated into cosmetics and sunscreens and are widely used in biomedical applications and the food industry. The increasing use of zinc oxide nanoparticles raises concerns about their safety. The aim of the study was to assess the effects of zinc oxide nanoparticles on oxidative and genotoxic parameters in the thyroid gland and liver of adult albino rats. Rats were divided into three groups; control, vehicle, and zinc oxide nanoparticles (200 mg/kg) and were subjected to treatment for 30 days. Oxidative stress parameters and genotoxicity were determined. Histopathological examination of both organs was undertaken. A significant reduction in triiodothyronine, thyroxine and thyroid-stimulating hormone was noted, whereas aspartate aminotransferase and alanine aminotransferase levels were significantly elevated. Increased malondialdehyde and decreased reduced glutathione levels were indicative of oxidative stress response in both organs. Additionally, elevated serum 8-hydroxydeoxyguanosine was noted which was supported by the results of the comet assay. Histopathological examination revealed alterations in the thyroid gland and liver. Sub-chronic exposure resulted in oxidative stress-mediated toxicity and genetic perturbations in both organs. Caution is warranted with repeated usage of products containing zinc oxide nanoparticles.

摘要

氧化锌纳米颗粒被掺入化妆品和防晒霜中，并广泛用于生物医学应用和食品工业。氧化锌纳米粒子的使用越来越多，引发了对其安全性的担忧。该研究的目的是评估氧化锌纳米颗粒对成年白化大鼠甲状腺和肝脏的氧化和遗传毒性参数的影响。大鼠分为三组；对照、载体和氧化锌纳米颗粒（200 mg/kg）并进行30天的处理。测定了氧化应激参数和遗传毒性。对两个器官进行了组织病理学检查。注意到三碘甲状腺原氨酸、甲状腺素和促甲状腺激素显着降低，而天冬氨酸氨基转移酶和丙氨酸氨基转移酶水平显着升高。丙二醛增加和谷胱甘肽水平降低表明两个器官中的氧化应激反应。此外，注意到升高的血清 8-羟基脱氧鸟苷，这得到了彗星试验结果的支持。组织病理学检查显示甲状腺和肝脏的改变。亚慢性暴露导致两个器官中的氧化应激介导的毒性和遗传扰动。重复使用含有氧化锌纳米颗粒的产品时要小心。亚慢性暴露导致两个器官中的氧化应激介导的毒性和遗传扰动。重复使用含有氧化锌纳米颗粒的产品时要小心。亚慢性暴露导致两个器官中的氧化应激介导的毒性和遗传扰动。重复使用含有氧化锌纳米颗粒的产品时要小心。