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Short note: Development of a new set of SNP markers to measure genetic diversity and genetic differentiation of Mongolian oak (Quercus mon­golica Fisch. ex Ledeb.) in the Far East of Russia
Silvae Genetica ( IF 1.1 ) Pub Date : 2019-01-01 , DOI: 10.2478/sg-2019-0016
Hilke Schröder 1 , Yulai Yanbaev 2 , Birgit Kersten 1 , Bernd Degen 1
Affiliation  

Abstract We developed a new set of 25 nuclear (nc), 12 chloroplast (cp) and 7 mitochondrial (mt) SNPs and used it to genotype 371 Mongolian oak (Quercus mongolica Fisch. ex Ledeb.) trees from seven locations in a 200 km by 400 km area in the Russian Far East. One of the locations in an area of 15 km by 25 km east of the city Ussuriusk was analyzed more intensively with 188 collected trees. The genetic differentiation at the nuclear SNPs was small to moderate and for the plastid SNPs it was high when considering all trees from the seven locations. The gene pool distances between locations were for 19 out of 21 pairs statistically highly significant. There was no correlation of genetic and spatial distances. Only three different multilocus-haplotypes could be identified and 42 two-loci-combinations of plastid SNPs could be used to identify them. Conclusions for the practical application such as timber tracking and gene conservation are discussed.

中文翻译:

简短说明:开发一组新的 SNP 标记来测量俄罗斯远东地区蒙古橡树 (Quercus mongolica Fisch. ex Ledeb.) 的遗传多样性和遗传分化

摘要 我们开发了一组新的 25 个核 (nc)、12 个叶绿体 (cp) 和 7 个线粒体 (mt) SNP,并用它对 200 公里范围内七个地点的 371 棵蒙古橡树 (Quercus mongolica Fisch. ex Ledeb.) 树进行基因分型。俄罗斯远东地区 400 公里。对乌苏里斯克市以东 15 公里乘 25 公里范围内的一个地点进行了更深入的分析,收集了 188 棵树木。核 SNP 的遗传分化是小到中等的,当考虑来自七个位置的所有树木时,对于质体 SNP,它是高的。位置之间的基因库距离对于 21 对中的 19 对具有统计学上的高度显着性。遗传距离和空间距离没有相关性。只能鉴定出三种不同的多位点单倍型,并且可以使用 42 个质体 SNP 的双位点组合来鉴定它们。
更新日期:2019-01-01
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