Abstract Koa (Acacia koa) and sub-species koaia (A. koaia) are two of more than 1,200 species from the genus Acacia within the Leguminosae (also designated Fabaceae) family. In the past, koa and koaia forests were found throughout the Hawaiian archipelago but populations have dramatically decreased. Comprehensive analyses of simple sequence repeats (SSRs) have not been published previously. Here we use genome sequencing and bioinformatics tools to report development of 100,000+ nuclear SSR (nuSSR) markers for use in koa and koaia genome studies with transcriptome SSR information was included for comparison. Over 10,000 high-value SSRs (40-60 % GC content) were isolated with 3,600+ further validated by ePCR. SSRs generated in this work can assist current efforts to sustainably increase in koa and koaia populations.

中文翻译：

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使用 Illumina 测序开发和验证 Acacia koa 和 A. koaia 核 SSR

摘要 Koa (Acacia koa) 和亚种 koaia (A. koaia) 是豆科（也称为豆科）中金合欢属的 1,200 多个物种中的两个。过去，整个夏威夷群岛都发现了 koa 和 koaia 森林，但种群数量急剧减少。之前尚未发表对简单序列重复 (SSR) 的综合分析。在这里，我们使用基因组测序和生物信息学工具报告了用于 koa 和 koaia 基因组研究的 100,000 多个核 SSR (nuSSR) 标记的开发，其中包括用于比较的转录组 SSR 信息。分离出超过 10,000 个高价值 SSR（40-60% GC 含量），其中 3,600+ 个通过 ePCR 进一步验证。在这项工作中产生的 SSR 可以帮助当前努力持续增加 koa 和 koaia 种群。