Abstract Wood constitutes the unique source of DNA in dead trees, but extraction of adequate quality DNA from dry wood is usually challenging. However, many different molecular studies require the use of such DNA. We have standardized and validated a modified CTAB protocol to isolate DNA from dry wood from Abies pinsapo and Cedrus atlantica species. Due to the degradation and very little DNA that is normally present in the wood from dead trees we have developed a PCR based test to certify the quality of the extracted samples. In the present study, we have proved too the effectiveness of this methodology to isolate DNA from conifer dry wood samples of sufficient quality to perform further molecular genetic experiments.

中文翻译：

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松科干木的DNA提取与扩增

摘要 木材是死树 DNA 的独特来源，但从干木材中提取足够质量的 DNA 通常具有挑战性。然而，许多不同的分子研究需要使用这种 DNA。我们已经标准化并验证了修改后的 CTAB 协议，以从冷杉和 Cedrus atlantica 物种的干木材中分离 DNA。由于枯死树的木材中通常存在降解和非常少的 DNA，我们开发了一种基于 PCR 的测试来证明提取样品的质量。在本研究中，我们也证明了这种方法可以有效地从具有足够质量的针叶树干木材样品中分离 DNA，以进行进一步的分子遗传实验。