This study demonstrated that a microbial community dominated by fungi can be selected and maintained in the long-term under non-sterile conditions, in a pilot-scale packed-bed reactor fed with tannery wastewater. During the start-up phase, the reactor, filled with 0.6 m³ of polyurethane foam cubes, was inoculated with a pure culture of Aspergillus tubingensis and Quebracho tannin, a recalcitrant compound widely used by tannery industry, was used as sole carbon source in the feeding. During the start-up, fungi grew attached as biofilm in carriers that filled the packed-bed reactor. Subsequently, the reactor was tested for the removal of chemical oxygen demand (COD) from an exhaust tanning bath collected from tanneries. The entire experiment lasted 121 days and average removals of 29% and 23% of COD and dissolved organic carbon (DOC) from the tannins bath were achieved, respectively. The evolution of the microbial consortium (bacteria and fungi) was described through biomolecular analyses along the experiment and also developed as a function of the size of the support media.

中文翻译：

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制革废水顽固化合物促进在非无菌条件下选择真菌：中试规模的长期试验

该研究表明，在非无菌条件下，在以制革废水供料的中试规模填充床反应器中，可以选择和长期维持以真菌为主的微生物群落。在启动阶段，用 0.6 m ³聚氨酯泡沫立方体填充的反应器接种塔宾曲霉和白斑曲霉的纯培养物单宁是制革工业广泛使用的顽固化合物，被用作饲料中的唯一碳源。在启动期间，真菌在填充填充床反应器的载体中以生物膜的形式附着生长。随后，测试反应器从制革厂收集的废鞣浴中去除化学需氧量 (COD)。整个实验持续了 121 天，分别从单宁浴中平均去除了 29% 和 23% 的 COD 和溶解有机碳 (DOC)。微生物聚生体（细菌和真菌）的进化是通过实验过程中的生物分子分析来描述的，并且还作为支持介质大小的函数而发展。