The pine wood nematode (PWN), Bursaphelenchus xylophilus, that causes pine wilt disease (PWD), has severely damaged forests and ecosystems in countries of East Asia and Europe. Since the high propagation rate of PWD is the main cause of the devastating disease, it is very important to interfere with the spread of PWN mediated by its insect vector (Monochamus alternatus). In order to explore new methods and approaches for the prevention and control of pine wood nematode disease, chemicals with attraction or repellency to pine wood nematode and stable diffusion or volatilization in infected wood were screened out to interfere and hinder the pupa chamber effect, the aggregation behavior of PWN to pupa chamber in M. alternatus. In the present study, 22 chemicals were inoculated on water agar medium and infected wood to verify the tropism of PWN toward the chemicals from the aspects of attraction and repellency. The results indicate that linoleic acid, oleic acid, diacetyl and β-myrcene have attractive effects on PWN, while kerosene and β-humulene have repellent effects on PWN and are able to stably permeate and volatilize within pine trees. This research shows that some chemicals can affect the movement behavior of PWN and interfere with its migration to the pupal chamber in M. alternatus, thus reducing the dispersal efficiency of PWNs carried by insect vectors. This chemically based strategy would provide novel approaches for effective controlling of pine wilt disease.

中文翻译：

---

几种化学物质对松材线虫（Steiner & Buhrer）镍迁移行为的影响

松材线虫 (PWN) 松材线虫 ( Bursaphelenchus xylophilus ) 导致松材枯萎病 (PWD)，严重破坏了东亚和欧洲国家的森林和生态系统。由于PWD的高繁殖率是造成这种破坏性疾病的主要原因，因此干扰由其昆虫载体（Monochamus alternatus）介导的PWN的传播非常重要。为探索防治松材线虫病的新方法和途径，筛选出对松材线虫具有吸引力或排斥性且在受侵染木材中稳定扩散或挥发的化学物质，干扰和阻碍蛹室效应、聚集体。 PWN 对M 中蛹室的行为。交替. 在本研究中，将22种化学品接种在水琼脂培养基和感染木材上，从吸引和排斥方面验证PWN对化学品的趋向性。结果表明，亚油酸、油酸、双乙酰和β-月桂烯对PWN具有诱人作用，而煤油和β-葎草烯对PWN具有驱避作用，能够在松树内稳定渗透和挥发。这项研究表明，一些化学物质会影响 PWN 的运动行为并干扰其向M 中的蛹室迁移。alternatus，从而降低昆虫载体携带的 PWNs 的传播效率。这种基于化学的策略将为有效控制松树枯萎病提供新方法。