Abstract

Aim

To investigate the effects of resveratrol on oxidative stress and inflammation parameters and histological alterations in cisplatin-induced optic nerve damage in a mouse model.

Material and Method

Thirty-six albino Wistar male rats were divided into three groups as control, 5 mg/kg cisplatin-administered (Cis) and 5 mg/kg cisplatin + 25 mg/kg resveratrol-administered (Cis + Res) animals. At the end of the experimental period, the rats were sacrificed with high-dose (50 mg/kg) thiopental sodium, and their optic nerves were dissected. Malondialdehyde (MDA), total glutathione (tGSH), total oxidant status (TOS), total antioxidant status (TAS), tumour necrosis factor alpha (TNF-α), nuclear factor kappa B (NF-KB) levels, and histopathological findings were assessed using the optic nerve tissues.

Results

In the Cis + Res group, the MDA, TOS, OSI, TNF-a and NFK-B levels were significantly lower and the tGSH and TAS levels were significantly higher compared with the Cis group (P = 0.001). In histological evaluations, there were dilated and congested blood vessels, destruction, oedema, degeneration, haemorrhage, and proliferating capillaries indicating the presence of inflammation and damage only in the Cis-administered group. However, in the Cis + Res group, the histological findings were very similar to the healthy controls.

Conclusion

Resveratrol is a promising neuroprotective agent for cisplatin-induced optic nerve toxicity with its anti-oxidant and anti-inflammatory effects. Further investigations are needed to evaluate the possible therapeutic effects on other optic nerve toxicities.

中文翻译：

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使用白藜芦醇防止实验性顺铂诱导的视神经毒性：大鼠模型研究

摘要

目标

研究白藜芦醇对小鼠模型中顺铂诱导的视神经损伤的氧化应激和炎症参数以及组织学改变的影响。

材料和方法

将 36 只白化 Wistar 雄性大鼠分为三组作为对照，5 mg/kg 顺铂给药 (Cis) 和 5 mg/kg 顺铂 + 25 mg/kg 白藜芦醇给药 (Cis + Res) 动物。实验结束时，用大剂量（50mg/kg）硫喷妥钠处死大鼠，解剖其视神经。丙二醛 (MDA)、总谷胱甘肽 (tGSH)、总氧化状态 (TOS)、总抗氧化状态 (TAS)、肿瘤坏死因子 α (TNF-α)、核因子 kappa B (NF-KB) 水平和组织病理学结果分别为使用视神经组织进行评估。

结果

Cis+Res组MDA、TOS、OSI、TNF-a、NFK-B水平显着低于Cis组，tGSH、TAS水平显着高于Cis组（P  =0.001）。在组织学评估中，仅在顺式给药组中存在扩张和充血的血管、破坏、水肿、退化、出血和毛细血管增殖，表明存在炎症和损伤。然而，在 Cis + Res 组中，组织学发现与健康对照组非常相似。

结论

白藜芦醇是一种很有前途的神经保护剂，可用于顺铂诱导的视神经毒性，具有抗氧化和抗炎作用。需要进一步研究来评估对其他视神经毒性的可能治疗效果。