The sorghum aphid (Melanaphis sacchari) has emerged as a serious pest for Sorghum bicolor and a major factor limiting its production worldwide. In this study, whole-genome resequencing of resistant and susceptible bulks from a recombinant inbred line (RIL) population was conducted together with transcriptome analysis of the parent to identify genes underlying aphid resistance. In the NGS-based bulked segregation analysis (BSA), using a total of 920,389 genome-wide high-confidence markers, four QTLs, qtlMs-6.1, qtlMs-6.2, qtlMs-6.3, and qtlMs-6.4, were identified on chromosome 6. Based on comparative transcriptome analysis, 245 differentially expressed genes (DEGs,190 upregulated and 55 downregulated) and 576 DEGs (336 upregulated and 240 downregulated) were identified at 4 dpi in 407B (resistant) and 7B (susceptible), respectively. The DEGs in 407B were enriched in ‘DNA replication’, ‘flavone and flavonol biosynthesis’ and ‘flavonoid biosynthesis’, which are associated with plant stress resistance. The QTL-seq study identified a SNP marker (Chr6: 2686447C>G) that was validated on a diverse panel and could be used in molecular marker-assisted selection to improve aphid resistance in sorghum.

中文翻译：

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全基因组重测序和转录组分析为双色高粱抗蚜虫数量性状位点/基因提供了见解

高粱蚜虫（Melanaphis sacchari）已成为双色高粱的严重害虫，也是限制其在世界范围内生产的主要因素。在这项研究中，对来自重组近交系 (RIL) 群体的抗性和易感群体的全基因组重测序与亲本的转录组分析一起进行，以鉴定潜在的蚜虫抗性基因。在基于 NGS 的批量分离分析 (BSA) 中，总共使用了 920,389 个全基因组高置信度标记，四个 QTL，qtlMs-6.1、qtlMs-6.2、qtlMs-6.3和qtlMs-6.4, 在 6 号染色体上鉴定。 基于比较转录组分析，在 4 dpi 的 407B（抗性）和 7B（敏感）， 分别。407B 中的 DEG 富含“DNA 复制”、“黄酮和黄酮醇生物合成”和“类黄酮生物合成”，这些与植物抗逆性有关。QTL-seq 研究确定了一个 SNP 标记（Chr6：2686447C>G），该标记在不同的面板上得到验证，可用于分子标记辅助选择，以提高高粱的蚜虫抗性。