ABSTRACT

The molecular identification of different taxa of genus Acacia plays a critical role to understand the evolutionary history of this important plant that has many medicinal and ecological benefits and distributed in many regions around the world (Africa, Asia, Australia and the Americas). This is the first study that used DNA barcoding (rbcL and matK) with 24 taxa of genus Acacia collected from Saudi Arabia for molecular identification, genetic resources preservation, detect genetic variations accompanied with a phylogenetic view and test the ability of DNA barcoding to segregate Senegalia and Vachellia from Acacia Mill taxa. Results showed that the GC ratio and transition/transversion bias (R) of rbcL (1.25) was higher than matK (0.95). This study resulted in the re-definition of some previously recorded species of Acacia into recently proposed genera,13 species under Vachellia and 2 species under Senegalia.The aim of the present study is to assess the ability of rbcL and matK loci to recognize Senegalia and Vachellia from other taxa of Acacia and assess the evolutionary relationship among them. This work will help to determine the position of Acacia collected from Saudi Arabia in the new generic classification and provide a clear view of the genetic relationships within the genera.

摘要

金合欢属不同分类群的分子鉴定对于了解这种具有许多药用和生态效益并分布于世界许多地区（非洲、亚洲、澳大利亚和美洲）的重要植物的进化历史起着至关重要的作用。这是第一项使用DNA条形码（rbcL和matK）对从沙特阿拉伯采集的金合欢属24个分类群进行分子鉴定、遗传资源保存、检测遗传变异和系统发育视图以及测试DNA条形码分离塞内加利亚的能力的研究和来自相思的瓦切利亚磨坊分类群。结果表明，rbcL (1.25)的 GC 比率和转换/颠换偏差 (R)高于matK (0.95)。该研究导致了一些先前记录的物种的重新定义相思到最近提出属，13种下Vachellia 2种下Senegalia .The目的本研究的是评估的能力rbcL基因和matK序列位点以识别Senegalia和来自金合欢其他分类群的Vachellia并评估它们之间的进化关系。这项工作将有助于确定金合欢的位置 从沙特阿拉伯收集的新属分类，并提供属内遗传关系的清晰视图。