Recent environmental problems caused by petroleum-based production of chemicals have accelerated the development of biorefineries for bio-based production of chemicals. Non-pathogenic microorganism, Corynebacterium glutamicum, has extensively been engineered and used as an industrial platform host strain for the commercial production of amino acids, such as l-lysine and L-glutamate. However, only recently has it been developed beyond its use for amino acid production. Recent advances in multiomics approaches, synthetic biology tools and metabolic engineering strategies have enabled the development of recombinant C. glutamicum into a versatile microbial cell factory for bio-based production of value-added platform chemicals and polymers by utilization of a broad range of biomass-derived sugars. In this review, we discuss the recent development of synthetic biology tools and techniques used for the enhancement of C. glutamicum’s ability to utilize renewable resources, specifically lignocellulosic biomass, for the production of platform chemicals with C4-C6 carbon backbone such as C4-isobutanol, 2,3-butanediol, C5-itaconic acid, 3-methyl-1-butanol, 2-methyl-1-butanol and C6-muconic acid.

最近由基于石油的化学品生产引起的环境问题加速了用于化学品生物生产的生物精炼厂的发展。非致病性微生物，谷氨酸棒杆菌，已经广泛地被工程化并用作工业平台宿主菌株用于商业生产氨基酸，如升赖氨酸和大号谷氨酸盐。然而，直到最近它才被开发超越用于氨基酸生产。多组学方法、合成生物学工具和代谢工程策略的最新进展使重组谷氨酸棒杆菌的开发成为可能进入多功能微生物细胞工厂，通过利用广泛的生物质衍生糖类以生物为基础生产增值平台化学品和聚合物。在这篇综述中，我们讨论了合成生物学工具和技术的最新发展，该工具和技术用于增强谷氨酸棒杆菌利用可再生资源的能力，特别是木质纤维素生物质，用于生产具有 C4-C6 碳骨架的平台化学品，如 C4-异丁醇、2,3-丁二醇、C5-衣康酸、3-甲基-1-丁醇、2-甲基-1-丁醇和C6-粘康酸。