Sugarcane molasses was examined for the production of poly(3-hydroxybutyrate) [P(3HB)] and poly(3-hydroxybutyrate-co-lactate) [P(3HB-co-LA)] in recombinant Ralstonia eutropha strains expressing Mannheimia succiniciproducens sacC gene encoding β-fructofuranosidase, which can hydrolyze sucrose into glucose and fructose in the culture medium. When crude sugarcane molasses was added to the culture medium to support 20 g/L of sucrose in flask cultivation, the growth of R. eutropha NCIMB11599 expressing the sacC gene was significantly inhibited, which resulted in OD₆₀₀ of 1.2 with P(3HB) content of 0.1wt%. The inhibition of cell growth due to the usage of the crude sugarcane molasses was relieved by pretreatment of sugarcane molasses with activated charcoal. Sugarcane molasses pretreated with activated charcoal could support the growth of R. eutropha NCIMB11599 expressing the sacC gene to OD600 of 87.2 with P(3HB) content of 82.5 wt% in batch fermentation when it was added to culture medium to support 20 g/L of sucrose. Also, R. eutropha 437–540 expressing Escherichia coli ldhA gene encoding lactate dehydrogenase along with the sacC gene produced P(3HB-co-6.2 mol%LA) with 29.1 wt% polymer content from sugarcane molasses in batch fermentation.

检测甘蔗糖蜜在表达Mannheimia succiniciproducens sacC 的重组真养产碱菌菌株中聚（3-羟基丁酸）[P(3HB)] 和聚（3-羟基丁酸-co-乳酸）[P(3HB - co -LA)] 的产量编码β-呋喃果糖苷酶的基因，可将培养基中的蔗糖水解成葡萄糖和果糖。当在培养瓶中加入粗制甘蔗糖蜜以支持 20 g/L 蔗糖时，表达sacC基因的R. eutropha NCIMB11599的生长受到显着抑制，导致 OD ₆₀₀1.2，P(3HB) 含量为 0.1wt%。通过用活性炭预处理甘蔗糖蜜，缓解了由于使用粗甘蔗糖蜜而导致的细胞生长抑制。用活性炭预处理的甘蔗糖蜜可以支持表达sacC基因的R. eutropha NCIMB11599的生长，OD600 为 87.2，分批发酵中 P(3HB) 含量为 82.5 wt%，当它添加到培养基中以支持 20 g/L 的蔗糖。此外，R. eutropha 437-540 表达编码乳酸脱氢酶的大肠杆菌 ldhA基因以及sacC基因，在分批发酵中从甘蔗糖蜜中产生 P(3HB -co -6.2 mol%LA) 聚合物含量为 29.1 wt%。