An experimental design was performed to study the combined effects of the parameters used for bacteria selective biofilm formation. The parameters were the potential applied, the time of the biofilm formation with electroactive bacteria or the biofilm formation, and the initial substrate concentration in the medium. The performance of the biofilms formed was compared by their kinetic parameter the apparent electron transfer rate constant (K_app), which was obtained from cyclic voltammetry analysis. The results from the experimental design showed that the highest K_app of 0.56 s⁻¹ was with the potential applied of −0.45 V for 5 h and 12 mM of the initial substrate concentration in the medium. New values of these parameters were calculated from the statistical analysis of the experimental design results and successfully higher value of K_app of 0.64 s⁻¹ can be calculated and obtained. The electroactivity and capability of the biofilm for hydrogen production was also proved in a single chamber microbial electrolysis cell, and a hydrogen production yield of 0.24 m³H₂/m³d was obtained. The biofilm formed with these last parameters was also identified with molecular techniques as Exiguobacterium which has barely been reported as electroactive bacteria and used for hydrogen production.

中文翻译：

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改进生物膜形成以应用于单室微生物电解池

进行了实验设计以研究用于细菌选择性生物膜形成的参数的组合效应。参数是施加的电位、用电活性细菌形成生物膜或生物膜形成的时间以及培养基中的初始底物浓度。形成的生物膜的性能通过它们的动力学参数进行比较，即表观电子转移速率常数 ( K _app )，这是从循环伏安法分析中获得的。实验设计的结果表明，0.56 s ^-1的最高K _app施加的电位为 -0.45 V，持续 5 小时，培养基中的初始底物浓度为 12 mM。这些参数的新值是从实验设计结果的统计分析中计算出来的，并且成功地计算并获得了 0.64 s ^{-1 的}更高K _app值。在单室微生物电解池中也证明了生物膜的电活性和产氢能力，并获得了0.24 m ³ H ₂ /m ³ d的产氢量。用这些最后的参数形成的生物膜也被分子技术鉴定为Exiguobacterium 它几乎没有被报道为电活性细菌并用于制氢。