Human samples offer attractive sources for disease diagnosis and biomarker discovery. In situ sampling and analysis of human samples is of paramount importance in the understanding of human physiological and pathological processes that take place in living human objects, and has been applied in various fields, including clinical diagnosis, forensic science, environmental health, biological chemistry, food and pharmaceutical science. Various strategies for coupling in situ solid phase microextraction with mass spectrometry (SPME-MS)-based approaches provide versatile methods to gain insight into human health. In this review, the developments and applications of in situ SPME-MS for detecting analytes from human exhaled breath, skin surface, and body fluids, are reviewed. Calibration methods and new features of in situ SPME-MS for onsite sampling and analysis of human metabolites are highlighted. The perspectives on further development and potential applications are also discussed.

人类样本为疾病诊断和生物标志物发现提供了有吸引力的来源。人体样本的原位采样和分析对于了解人体活体物体中发生的人体生理和病理过程至关重要，并已应用于各个领域，包括临床诊断、法医学、环境健康、生物化学、食品和药物科学。将原位固相微萃取与基于质谱 (SPME-MS) 的方法相结合的各种策略提供了多种方法来深入了解人类健康。在这篇综述中，原位技术的发展和应用回顾了用于检测来自人类呼出气、皮肤表面和体液的分析物的 SPME-MS。重点介绍了用于现场采样和分析人体代谢物的原位SPME-MS 的校准方法和新功能。还讨论了进一步开发和潜在应用的前景。