An evaluation of the impact of vitamin E deficiency on expression of the alpha-tocopherol transfer protein (α-TTP) and related CRAL_TRIO genes was undertaken using livers from adult zebrafish based on the hypothesis that increased lipid peroxidation would modulate gene expression. Zebrafish were fed either a vitamin E sufficient (E+) or deficient (E–) diet for 9 months, then fish were euthanized, and livers were harvested. Livers from the E+ relative to E– fish contained 40-times more α-tocopherol (P <0.0001) and one fourth the malondialdehyde (P = 0.0153). RNA was extracted from E+ and E– livers, then subject to evaluation of gene expression of ttpa and other genes of the CRAL_TRIO family, genes of antioxidant markers, and genes related to lipid metabolism. Ttpa expression was not altered by vitamin E status. However, one member of the CRAL_TRIO family, tyrosine-protein phosphatase non-receptor type 9 gene (ptpn9a), showed a 2.4-fold increase (P = 0.029) in E– relative to E+ livers. Further, we identified that the gene for choline kinase alpha (chka) showed a 3.0-fold increase (P = 0.010) in E– livers. These outcomes are consistent with our previous findings that show vitamin E deficiency increased lipid peroxidation causing increases in phospholipid turnover.

基于脂质过氧化增加会调节基因表达的假设，使用成年斑马鱼的肝脏评估了维生素 E 缺乏对 α-生育酚转移蛋白 (α-TTP) 和相关 CRAL_TRIO 基因表达的影响。斑马鱼被喂食维生素 E 充足 (E+) 或维生素 E 缺乏 (E–) 的饮食 9 个月，然后对鱼实施安乐死，并收获肝脏。 E+ 鱼的肝脏中的 α-生育酚含量是 E- 鱼的 40 倍（ P <0 id=109>P = 0.0153）。从E+和E-肝脏中提取RNA，然后评估ttpa和CRAL_TRIO家族其他基因、抗氧化标记基因以及脂质代谢相关基因的基因表达。 Ttpa表达不因维生素 E 状态而改变。然而，CRAL_TRIO 家族的一个成员，酪氨酸蛋白磷酸酶非受体 9 型基因 ( ptpn9a )，在 E– 肝脏中相对于 E+ 肝脏显示出 2.4 倍的增加 ( P = 0.029)。此外，我们还发现 E- 肝脏中的胆碱激酶 α ( chka ) 基因增加了 3.0 倍 ( P = 0.010)。这些结果与我们之前的研究结果一致，即维生素 E 缺乏会增加脂质过氧化，导致磷脂周转增加。