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Agro-morphological variability and genetic diversity in linseed (Linum usitatissimum L.) germplasm accessions with emphasis on flowering and maturity time
Genetic Resources and Crop Evolution ( IF 1.6 ) Pub Date : 2021-06-11 , DOI: 10.1007/s10722-021-01231-3
Ankit Saroha , Deepa Pal , Vikender Kaur , Sandeep Kumar , Arti Bartwal , J. Aravind , J. Radhamani , S. Rajkumar , Rajesh Kumar , Sunil S. Gomashe , Abhishek Sengupta , Dhammaprakash Pandhari Wankhede

Assessment of genetic diversity and extent of trait variation among germplasm accessions facilitate the effective use of genetic resources for varietal development. In linseed (Linum usitatissimum L.), early maturity is desirable as it helps avoid several biotic and abiotic stress. In present study, genetic variability of 220 accessions of linseed was studied using 25 agro-morphological traits for 2 years, 2017–2018 and 2018–2019. Remarkable variability was observed for flowering, maturity and other economically important traits such as thousand seed weight, capsule number, seed, and capsule area. Based on 2-year evaluation data for flowering and maturity traits, a panel of 34 accessions was constructed to study genetic diversity using SSR markers. Total 89 alleles were amplified with a mean of 3.069 alleles per locus. Polymorphism information content value ranged from 0.20 to 0.66 with mean of 0.42. Clustering based on SSR data followed the flowering-maturity trait pattern to a large extent. Genetic population structure analysis showed two populations in similarity with the trait groups. Analysis of molecular variance revealed 16% variance among the populations. Shannon's diversity information index within population I and II were 0.67 and 0.78, respectively. Additionally, allelic variation in a putative floral homeotic gene encoding APETALA2 transcription factor was studied in two early and two late accessions by PCR amplification and sequencing. Total 12 SNPs were identified, of which six could distinguish early and late accessions. The genetic and genomic resources identified here are expected to aid in linseed genetic improvement programme targeted to specific traits, especially, early flowering and maturity.



中文翻译:

以开花和成熟时间为重点的亚麻籽(Linum usitatissimum L.)种质资源的农业形态变异性和遗传多样性

种质种质间遗传多样性和性状变异程度的评估有助于有效利用遗传资源进行品种发育。在亚麻籽 ( Linum usitatissimumL.),早熟是可取的,因为它有助于避免几种生物和非生物胁迫。在本研究中,2017-2018 年和 2018-2019 年的 2 年间,使用 25 个农业形态性状研究了 220 份亚麻籽的遗传变异性。观察到开花、成熟和其他重要经济性状(如千粒重、蒴果数、种子和蒴果面积)的显着变异。基于开花和成熟性状的 2 年评估数据,构建了一个由 34 个种质组成的面板,以使用 SSR 标记研究遗传多样性。总共扩增了 89 个等位基因,每个基因座平均有 3.069 个等位基因。多态性信息含量值范围为0.20~0.66,均值为0.42。基于 SSR 数据的聚类在很大程度上遵循开花-成熟性状模式。遗传种群结构分析显示两个种群与性状群相似。分子方差分析显示群体间有 16% 的变异。种群 I 和 II 中香农的多样性信息指数分别为 0.67 和 0.78。此外,通过 PCR 扩增和测序在两个早期和两个晚期种质中研究了编码 APETALA2 转录因子的假定花卉同源异型基因的等位基因变异。共鉴定出 12 个 SNP,其中 6 个可以区分早期和晚期种质。预计这里确定的遗传和基因组资源将有助于针对特定性状,尤其是早花和成熟的亚麻籽遗传改良计划。分子方差分析显示种群间有 16% 的变异。种群 I 和 II 中香农的多样性信息指数分别为 0.67 和 0.78。此外,通过 PCR 扩增和测序在两个早期和两个晚期种质中研究了编码 APETALA2 转录因子的假定花卉同源异型基因的等位基因变异。共鉴定出 12 个 SNP,其中 6 个可以区分早期和晚期种质。预计这里确定的遗传和基因组资源将有助于针对特定性状,尤其是早花和成熟的亚麻籽遗传改良计划。分子方差分析显示种群间有 16% 的变异。种群 I 和 II 中香农的多样性信息指数分别为 0.67 和 0.78。此外,通过 PCR 扩增和测序在两个早期和两个晚期种质中研究了编码 APETALA2 转录因子的假定花卉同源异型基因的等位基因变异。共鉴定出 12 个 SNP,其中 6 个可以区分早期和晚期种质。预计这里确定的遗传和基因组资源将有助于针对特定性状,尤其是早花和成熟的亚麻籽遗传改良计划。通过 PCR 扩增和测序,在两个早期和两个晚期种质中研究了编码 APETALA2 转录因子的假定花卉同源异型基因的等位基因变异。共鉴定出 12 个 SNP,其中 6 个可以区分早期和晚期种质。预计这里确定的遗传和基因组资源将有助于针对特定性状,尤其是早花和成熟的亚麻籽遗传改良计划。通过 PCR 扩增和测序,在两个早期和两个晚期种质中研究了编码 APETALA2 转录因子的假定花卉同源异型基因的等位基因变异。共鉴定出 12 个 SNP,其中 6 个可以区分早期和晚期种质。预计这里确定的遗传和基因组资源将有助于针对特定性状,尤其是早花和成熟的亚麻籽遗传改良计划。

更新日期:2021-06-11
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