Routine wastewater treatment plants (WWTPs) effluents monitoring is essential because of enteric viruses’ low infectious dose beyond molecular detectability. In current study methods for concentration and extraction, inter-method compatibility and recovery efficiency of spiked human adenovirus (HAdV) and human rotavirus A (RVA) were evaluated. For virus concentration, polyethylene glycol precipitation (PEG), charged membrane-based adsorption/elution (CMAE), and glass wool-based concentration (GW) methods were used. Nucleic acid was extracted by PowerViral™ Environmental RNA/DNA Isolation (POW), ZymoBIOMICS™ RNA extraction (ZYMO) and Wizard® Genomic DNA Purification (WGDP) and samples were analyzed by Real-Time PCR. CMAE method yielded significantly higher concentrations for both ARQ (Armored-RNA Quant) and RVA compared to PEG (P = 0.001 and 0.003) and GW (P < 0.0001). Highest HAdV concentration was obtained by PEG (P = 0.001 and < 0.0001) in relation to CMAE and GW. ZYMO yielded a significantly higher ARQ and RVA concentrations (P = 0.03 and 0.0057), whereas significantly higher concentration was obtained by POW for HAdV (P = 0.032). CMAE × ZYMO achieved the highest recovery efficiencies for ARQ (69.77 %) and RVA (64.25, respectively, while PEG × POW present efficiency of 9.7 % for HAdV. These findings provide guidance for understanding of method-related biases for viral recovery efficiency.

常规污水处理厂 (WWTP) 流出物监测至关重要，因为肠道病毒的感染剂量低，超出​​分子可检测性。在当前的浓缩和提取研究方法中，评估了加标人腺病毒 (HAdV) 和人轮状病毒 A (RVA) 的方法间兼容性和回收效率。对于病毒浓缩，使用了聚乙二醇沉淀 (PEG)、基于带电的膜吸附/洗脱 (CMAE) 和基于玻璃棉的浓缩 (GW) 方法。通过 PowerViral™ 环境 RNA/DNA 分离 (POW)、ZymoBIOMICS™ RNA 提取 (ZYMO) 和 Wizard® 基因组 DNA 纯化 (WGDP) 提取核酸，并通过实时 PCR 分析样品。与 PEG 相比，CMAE 方法产生了显着更高的 ARQ（Armored-RNA Quant）和 RVA 浓度（P  = 0.001 和 0.003) 和 GW ( P  < 0.0001)。 与 CMAE 和 GW 相关的最高 HAdV 浓度是通过 PEG 获得的（P = 0.001 和 < 0.0001）。ZYMO 产生显着更高的 ARQ 和 RVA 浓度（P  = 0.03 和 0.0057），而通过 POW 获得显着更高的 HAdV 浓度（P  = 0.032）。CMAE × ZYMO 对 ARQ (69.77 %) 和 RVA (分别为 64.25) 的回收效率最高，而 PEG × POW 对 HAdV 的回收效率为 9.7 %。这些发现为理解病毒回收效率的方法相关偏差提供了指导。