Ostreopsis cf. ovata is a benthic and ovatoxin-producing dinoflagellate proliferating yearly along the Mediterranean coasts where blooms have been related to human illness and unusual mortality of marine organisms. The spreading of O. cf. ovata in this temperate area has been linked to global changes and its consequences such as the increase of temperature or light intensities. In the present study, an experimental design using batch cultures of pre-acclimated cells of a strain of O. cf. ovata isolated from Villefranche-sur-Mer (NW Mediterranean Sea, France), was implemented to investigate the combined effect of temperature (23, 27 and 30 °C) and light intensity (200, 400 and 600 µmol m⁻² s⁻¹) on the growth, metabolome and OVTX content. Both light intensity and temperature affected the growth as significantly higher growth rates were obtained under 400 and 600 µmol m⁻² s⁻¹ while the maximum values were obtained at 27 °C (0.48 d⁻¹). Metabolomic analyses highlighted a clear effect only for temperature that may correspond to two different strategies of acclimation to suboptimal temperatures. Significant features (such as carotenoid and lipids) modified by the temperature and/or light conditions were annotated. Only temperature induced a significant change of OVTX content with higher values measured at the lowest temperature of 23 °C (29 – 36 pg cell⁻¹). In a context of global changes, these results obtained after acclimation suggest that the increase of temperature might favor the proliferation of less toxic cells. However, in the light of the intraspecific variability of O. cf. ovata, further studies will be necessary to test this hypothesis. This study also highlighted the lack of knowledge about the metabolome composition of such non-model organisms that impairs data interpretation. There is a need to study more deeply the metabolome of toxic dinoflagellates to better understand how they can acclimate to a changing environment.

Ostreopsis参见。ovata是一种底栖和产卵毒素的甲藻，每年在地中海沿岸繁殖，那里的水华与人类疾病和海洋生物的异常死亡有关。O. cf.的传播。这个温带地区的卵与全球变化及其后果有关，例如温度或光照强度的增加。在本研究中，使用O. cf.菌株的预驯化细胞分批培养的实验设计。从 Villefranche-sur-Mer（法国西北部地中海）分离的ovata用于研究温度（23、27 和 30 °C）和光强度（200、400 和 600 µmol）的综合影响m ^-2 s ^-1 ) 对生长、代谢组和 OVTX 含量的影响。光强度和温度都影响生长，因为在 400 和 600 µmol m ^-2 s ^-1下获得了显着更高的生长速率，而最大值在 27°C (0.48 d ^-1 ) 下获得。代谢组学分析突出了仅对温度的明显影响，这可能对应于适应次优温度的两种不同策略。注释了由温度和/或光照条件修改的显着特征（例如类胡萝卜素和脂质）。只有温度导致 OVTX 含量发生显着变化，在 23 °C 的最低温度（29 – 36 pg 电池^-1）。在全球变化的背景下，驯化后获得的这些结果表明，温度的升高可能有利于毒性较小的细胞的增殖。然而，鉴于O. cf.的种内变异性。ovata，需要进一步的研究来检验这个假设。这项研究还强调了对此类非模式生物的代谢组组成的了解不足，这会影响数据解释。需要更深入地研究有毒鞭毛藻的代谢组，以更好地了解它们如何适应不断变化的环境。