Relationships between glomalin-related soil protein (GRSP) and soil organic carbon, total nitrogen or enzyme activity are unknown under the combined effects of varying daytime/nighttime elevated CO₂ (eCO₂) levels and arbuscular mycorrhizal fungi (AMF). We demonstrated that daytime, nighttime, and dual daytime/nighttime eCO₂ levels could significantly increase AMF mycorrhization, soil organic carbon, total nitrogen, easily extractable, difficultly extractable and total GRSP in AMF soil. Under daytime eCO₂, soil total nitrogen was significantly decreased in non-AMF soil, while mycorrhization alleviated soil nitrogen limitation. Nighttime and dual daytime/nighttime eCO₂ significantly increased β-glucosidase and protease activities in AMF and non-AMF soils. With greater eCO₂ effects in AMF than in non-AMF soils, GRSPs positively related to mycorrhization, soil organic carbon, total nitrogen, and β-glucosidase activity. Under the circumstance of global CO₂ rising, besides mycorrhization, the combined effects of varying, non-steady-state daytime and nighttime eCO₂, play important roles in soil carbon and nitrogen cycles.

在不同白天/夜间升高的 CO ₂ (eCO ₂ ) 水平和丛枝菌根真菌 (AMF)的综合影响下，球藻素相关土壤蛋白 (GRSP) 与土壤有机碳、总氮或酶活性之间的关系尚不清楚。我们证明白天、夜间和双白天/夜间 eCO ₂水平可以显着增加 AMF 土壤中的 AMF 菌根、土壤有机碳、总氮、易提取、难提取和总 GRSP。在白天eCO _{2 下}，非AMF土壤的土壤总氮显着降低，而菌根作用缓解了土壤氮限制。夜间和双白天/夜间 eCO ₂显着增加 AMF 和非 AMF 土壤中 β-葡萄糖苷酶和蛋白酶的活性。与非 AMF 土壤相比，AMF 中的eCO ₂效应更大，GRSP 与菌根、土壤有机碳、总氮和 β-葡萄糖苷酶活性呈正相关。在全球CO ₂上升的情况下，除了菌根作用外，白天和夜间变化的、非稳态的eCO _{2 的综合}作用在土壤碳和氮循环中起着重要作用。