The aims of the presented study were the investigation of the effect of different ultrasound-assisted extraction conditions (particle size, solid-to-solvent ratio, ethanol concentration and time) on polyphenol and flavonoid contents and antioxidant activity of wild thyme (Thymus serpyllum L.) extracts, HPLC qualitative and quantitative analysis, as well as the examination of antimicrobial properties against seven bacterial strains and one fungal strain. According to the results, the highest total polyphenol and flavonoid contents were reached with particle size of 0.3 mm, 1:30 solid-to-solvent ratio, 30 % ethanol and 30 min of extraction (31.9 ± 0.1 mg gallic acid equivalents (GAE)/L and 16.8 ± 0.2 mg catechin equivalents (CE)/L, respectively). The most abundant polyphenolic compounds were rosmarinic acid, luteolin 7-O-glucuronide and salvianolic acid I (185.2 ± 7.3, 141.6 ± 3.4 and 76.4 ± 2.1 μg/mL, respectively). The best antioxidant recovery was achieved with particles of 0.3 mm and 1:30 solid-to-solvent ratio. Furthermore, the addition of ethanol in aqueous extraction medium has caused significantly better antioxidant activity than pure water. On the other hand, there was no statistically significant difference in antioxidant capacity between various ethanol percentage, as well as between time of 15 and 30 min. Selected extracts have shown significant antibacterial activity, particularly against Enterococcus faecalis, Bacillus cereus, Listeria monocytogenes, Staphylococcus aureus and Yersinia enterocolitica.

本研究的目的是研究不同的超声辅助提取条件（粒径、固溶比、乙醇浓度和时间）对野生百里香 ( Thymus serpyllum ) 的多酚和黄酮含量以及抗氧化活性的影响。L.) 提取物、HPLC 定性和定量分析，以及对七种细菌菌株和一种真菌菌株的抗菌特性的检查。根据结果​​，当粒径为 0.3 mm、固溶剂比为 1:30、乙醇含量为 30% 且提取时间为 30 分钟（31.9 ± 0.1 mg 没食子酸当量 (GAE)）时，总多酚和类黄酮含量达到最高/L 和 16.8 ± 0.2 mg 儿茶素当量 (CE)/L）。最丰富的多酚化合物是迷迭香酸、木犀草素 7- O-葡萄糖醛酸和丹酚酸 I（分别为 185.2 ± 7.3、141.6 ± 3.4 和 76.4 ± 2.1 μg/mL）。使用 0.3 毫米的颗粒和 1:30 的固溶剂比实现了最佳的抗氧化剂回收率。此外，在水性提取介质中添加乙醇已导致比纯水明显更好的抗氧化活性。另一方面，不同乙醇百分比之间以及 15 和 30 分钟之间的抗氧化能力没有统计学上的显着差异。选定的提取物显示出显着的抗菌活性，特别是对粪肠球菌、蜡状芽孢杆菌、单核细胞增生李斯特菌、金黄色葡萄球菌和小肠结肠炎耶尔森氏菌。