Background Circular RNA (circRNA) plays an essential role in tumor progression, including glioma. circ_0030018 is a newly discovered circRNA that is highly expressed in glioma. However, its role and mechanism in glioma need to be further elucidated. Methods The expression of circ_0030018, microRNA (miR)-194-5p, and tripartite motif containing 44 (TRIM44) was examined using quantitative real-time PCR. Cell proliferation, migration, invasion, and apoptosis were determined using MTT assay, colony formation assay, transwell assay, and flow cytometry. Moreover, dual-luciferase reporter assay and RNA pull-down assay were used to verify the interactions among circ_0030018, miR-194-5p, and TRIM44 . The protein expression of TRIM44 was assessed by western blot analysis. Animal experiments were conducted to explore the role of circ_0030018 in glioma tumor growth in vivo . Results circ_0030018 was overexpressed in glioma tissues and cells, and its silencing could inhibit glioma cell proliferation, migration, invasion, and accelerate apoptosis. miR-194-5p could be sponged by circ_0030018, and its overexpression could hinder the progression of glioma cells. Further experiments revealed that miR-194-5p inhibitor reversed the negative regulation of circ_0030018 knockdown on glioma cell progression. In addition, TRIM44 was a target of miR-194-5p, and its downregulation could repress glioma cell progression. Overexpressed TRIM44 reversed the inhibition effect of miR-194-5p on glioma cell progression. Animal experiments suggested that circ_0030018 knockdown could reduce glioma tumor growth through regulating miR-194-5p and TRIM44. Conclusion Our 8data showed that circ_0030018 enhanced glioma progression by sponging miR-194-5p to regulate TRIM44, indicating that circ_0030018 might be a potential treatment target for glioma.

中文翻译：

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circ_0030018 促进胶质瘤增殖和转移

背景 环状 RNA (circRNA) 在包括胶质瘤在内的肿瘤进展中发挥着重要作用。circ_0030018是新发现的一种在胶质瘤中高表达的circRNA。然而，其在胶质瘤中的作用和机制有待进一步阐明。方法 使用实时定量 PCR 检测 circ_0030018、microRNA (miR)-194-5p 和含有 44 的三方基序 (TRIM44) 的表达。使用MTT测定、集落形成测定、transwell测定和流式细胞术测定细胞增殖、迁移、侵袭和凋亡。此外，使用双荧光素酶报告基因分析和 RNA pull-down 分析验证 circ_0030018、miR-194-5p 和 TRIM44 之间的相互作用。通过蛋白质印迹分析评估TRIM44的蛋白质表达。进行动物实验，探讨circ_0030018在体内胶质瘤肿瘤生长中的作用。结果circ_0030018在胶质瘤组织和细胞中过表达，其沉默可抑制胶质瘤细胞增殖、迁移、侵袭，加速细胞凋亡。miR-194-5p 可以被 circ_0030018 吸收，其过表达可能会阻碍胶质瘤细胞的进展。进一步的实验表明，miR-194-5p 抑制剂逆转了 circ_0030018 敲低对胶质瘤细胞进展的负调控。此外，TRIM44 是 miR-194-5p 的靶点，其下调可以抑制胶质瘤细胞的进展。过表达的 TRIM44 逆转了 miR-194-5p 对胶质瘤细胞进展的抑制作用。动物实验表明，circ_0030018 敲低可以通过调节 miR-194-5p 和 TRIM44 来减少胶质瘤的生长。结论 我们的 8 个数据显示 circ_0030018 通过海绵状 miR-194-5p 调节 TRIM44 促进胶质瘤进展，表明 circ_0030018 可能是胶质瘤的潜在治疗靶点。