Evidence has shown that oestrogen suppresses lipids deposition in the liver of mammals. However, the molecular mechanism of oestrogen action in hepatic steatosis of geese liver has yet to be determined. This study aimed to investigate the effect of oestrogen on lipid homeostasis at different states of geese hepatocytes in vitro. The results showed that an in vitro model of hepatic steatosis was induced by 1.5 mM sodium oleate via detecting the viability of hepatocytes and content of lipids. When the normal hepatocytes were administrated with different concentrations of oestrogen (E₂), the expression levels of diacylglycerol acyltransferase 2 (DGAT2), microsomal triglyceride transfer protein (MTTP) and oestrogen receptors (ERs, alpha and beta) were up-regulated only at high concentrations of E₂, whereas the lipid content was not a significant difference. In goose hepatocytes of hepatic steatosis, however, the expression levels of MTTP, apolipoprotein B (apoB) and ERα/β significantly increased at 10⁻⁷ or 10⁻⁶ M E₂. Meanwhile, the lipids content significantly increased at 10⁻⁹ and 10⁻⁸ M E₂ and decreased at 80 µM E₂. Further heatmap analysis showed that ERα was clustered with apoB and MTTP in either normal hepatocytes or that of hepatic steatosis. Taken together, E₂ might bind to ERα to up-regulate the expression levels of apoB and MTTP, promoting the transportation of lipids and alleviating lipids overload in hepatic steatosis of geese in vitro.

中文翻译：

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雌激素通过雌激素受体α促进鹅肝脂肪变性体外脂质转运

有证据表明，雌激素抑制哺乳动物肝脏中的脂质沉积。然而，雌激素作用于鹅肝脂肪变性的分子机制尚待确定。本研究旨在探讨雌激素对体外不同状态鹅肝细胞脂质稳态的影响。结果表明，通过检测肝细胞活力和脂质含量，1.5 mM油酸钠可诱导肝脏脂肪变性的体外模型。正常肝细胞给予不同浓度的雌激素（E ₂）时，甘油二酯酰基转移酶2（DGAT2）、微粒体甘油三酯转移蛋白（MTTP）和雌激素受体的表达水平（ERs 、α和β）仅在高浓度的E ₂时上调，而脂质含量没有显着差异。然而，在肝脂肪变性的鹅肝细胞中，MTTP、载脂蛋白B( apoB )和ERα / β的表达水平在10 ^-7或10 ^-6  ME ₂显着增加。同时，脂质含量在 10 ^-9和 10 ^-8  ME ₂显着增加，在 80 µME ₂下降。进一步的热图分析表明，ERα与apoB和MTTP在正常肝细胞或肝脂肪变性的肝细胞中。综上所述，E ₂ 可能与ERα结合，上调apoB和MTTP的表达水平，促进脂类转运，缓解体外鹅肝脂肪变性的脂类超载。