ABSTRACT

Introduction: In this study, we aimed to evaluate the hepatic protective effects of dexmedetomidine in the lower extremity ischemia-reperfusion model in diabetic rats biochemically and histopathologically.

Methods: Rats were randomly divided into 4 equal groups (n = 6); Control (C) group, diabetic control group (DM), diabetic ischemia-reperfusion group (IR), group with diabetic IR and dexmedetomidine (DEX). In the IR and DEX groups were performed 120 min reperfusion after 120 min ischemia. In group DEX, 100 µ / kg dexmedetomidine was administered intraperitoneally 30 minutes before renal IR administration. Then, various histopathological and biochemical parameters were evaluated in liver tissue.

Results: After ischemia-reperfusion, aspartate amino transaminase, alanine amino transaminase, total oxidant level, and thiobarbituric acid -reactive substances were increased, total thiol group and total antioxidant level were decreased and these parameters were found to improve in the group given dexmedetomidine. It was also observed that there was histopathological deterioration after ischemia-reperfusion and histopathological deterioration was found to be less with dexmedetomidine administration.

Conclusion: The effects of lower extremity ischemia-reperfusion on hepatic tissue as distant organs were evaluated in diabetic rats, histopathologically, immunologically, biochemically, and liver damage was determined after ischemia-reperfusion, and dexmedetomidine was found to decrease liver damage.

摘要

介绍：在本研究中，我们旨在从生化和组织病理学方面评估右美托咪定在糖尿病大鼠下肢缺血再灌注模型中的肝脏保护作用。

方法：将大鼠随机分为4组（n=6）；对照组(C)、糖尿病对照组(DM)、糖尿病缺血再灌注组(IR)、糖尿病IR和右美托咪定(DEX)组。IR和DEX组在缺血120分钟后进行120分钟再灌注。在DEX组中，在肾IR给药前30分钟腹膜内给药100μ/kg右美托咪定。然后，在肝组织中评估了各种组织病理学和生化参数。

结果：缺血再灌注后，右美托咪定组天冬氨酸氨基转氨酶、丙氨酸氨基转氨酶、总氧化剂水平和硫代巴比妥酸反应物水平升高，总硫醇组和总抗氧化剂水平降低，这些参数有所改善。还观察到缺血再灌注后组织病理学恶化，并且发现给予右美托咪定的组织病理学恶化较少。

结论：通过组织病理学、免疫学、生化和缺血再灌注后的肝损伤评估了糖尿病大鼠下肢缺血再灌注对远器官肝组织的影响，发现右美托咪定可减轻肝损伤。