Abstract

Structured lipid microparticles (StLMs) composed of oil and solid lipid in specific ratios are drawing significant attention, owing to their stability in loading and controllability in releasing bioactive compounds. In this study, we formulated monodisperse StLMs with palmitic acid or tripalmitin mixed with soybean oil in different ratios using microchannel emulsification (MCE) at ∼70 °C and following crystallization by cooling. Based on the physicochemical properties of the different dispersed phases, the formulation characteristics were observed and investigated using a microscope video system connected to the MCE system. For each ratio and composition at ∼70 °C, the lipid droplets presented average diameter and coefficient of variation (CV) values in respective ranges of 15.5–19.0 μm and 3.3–8.4%. Following droplet crystallization by cooling, the StLMs exhibited morphological changes but negligible changes in average size and CV. From the internal structure of each lipid molecular and the fundamental information supplied by differential scanning calorimetry (DSC) experiments, we outline the assumptions for morphology variation, which can be used as reference for encapsulation research of StLMs.

摘要

由特定比例的油和固体脂质组成的结构化脂质微粒（StLMs）由于其负载稳定性和释放生物活性化合物的可控性而引起了广泛关注。在这项研究中，我们使用微通道乳化 (MCE) 在约 70 °C 下和冷却结晶后，将棕榈酸或三棕榈酸与大豆油以不同比例混合制成单分散 StLMs。基于不同分散相的物理化学性质，使用连接到MCE系统的显微镜视频系统观察和研究配方特性。对于~70°C 下的每个比例和组成，脂滴的平均直径和变异系数 (CV) 值分别在 15.5-19.0 μm 和 3.3-8.4% 的范围内。通过冷却液滴结晶后，StLMs 表现出形态变化，但平均大小和 CV 的变化可以忽略不计。从每个脂质分子的内部结构和差示扫描量热法（DSC）实验提供的基本信息，我们概述了形态变化的假设，可以作为StLMs封装研究的参考。