Wound healing is a complicated process constituted of four successive physiological stages involving wound bleeding, inflammatory response, cell proliferation and tissue remodeling. During this period, bacteria can easily infect the wound. Therefore, we prepared a novel curcumin-loaded sandwich-like nanofibrous membrane (CSNM) using sequential electrospinning for the hemostasis, antibacterial and accelerate wound healing. The morphology of the nanofibrous membrane was analyzed by SEM. In addition, the water absorption capacity, water vapor transmission rate, water contact-angle, and in vitro drug release were all tested. Then in vitro and in vivo hemostatic experiments demonstrated that CSNM has a good hemostatic effect. Antioxidant effect was assessed by the DPPH radical scavenging method and CSNM presented a high antioxidant activity. Additionally, CSNM demonstrated excellent antibacterial activity by the disk diffusion method. Furthermore, the rat dorsal skin defects model revealed that the CSNM distinctly induced the granulation tissue grew, collagen deposition and epithelial tissue remodeling. Meanwhile, the results of the immunohistochemical staining showed that the CSNM can facilitate the expression of CD31 and TGF-β in the early stage of the wound, thereby accelerating wound healing. In general, this study proved that the multifunctional CSNM has great potential as wound dressing in wound healing.

伤口愈合是一个复杂的过程，由四个连续的生理阶段组成，包括伤口出血、炎症反应、细胞增殖和组织重塑。在此期间，细菌很容易感染伤口。因此，我们使用顺序静电纺丝制备了一种新型的负载姜黄素的三明治状纳米纤维膜（CSNM），用于止血、抗菌和加速伤口愈合。通过SEM分析纳米纤维膜的形态。此外，还测试了吸水能力、水蒸气透过率、水接触角和体外药物释放。然后体外和体内止血实验证明CSNM具有良好的止血效果。通过DPPH自由基清除方法评估抗氧化作用，CSNM表现出高抗氧化活性。此外，CSNM 通过圆盘扩散法表现出优异的抗菌活性。此外，大鼠背部皮肤缺损模型显示，CSNM明显诱导肉芽组织生长、胶原沉积和上皮组织重塑。同时，免疫组化染色结果表明，CSNM可以促进创面早期CD31和TGF-β的表达，从而加速创面愈合。总的来说，这项研究证明多功能CSNM在伤口愈合中作为伤口敷料具有巨大的潜力。免疫组化染色结果表明，CSNM可以促进创面早期CD31和TGF-β的表达，从而加速创面愈合。总的来说，这项研究证明多功能CSNM在伤口愈合中作为伤口敷料具有巨大的潜力。免疫组化染色结果表明，CSNM可以促进创面早期CD31和TGF-β的表达，从而加速创面愈合。总的来说，这项研究证明多功能CSNM在伤口愈合中作为伤口敷料具有巨大的潜力。