In recent years, organic fertilizer (OF) instead of chemical fertilizer is an effective way to improve the utilization efficiency of crop resources and the quality of agricultural products. However, knowledge of the effects of OF on soil microorganisms is still limited. The microbial communities in two soils of Yinchuan (P) and Guyuan (T) city were studied by high-throughput sequencing, and the correlation between their community structure and soil properties was analyzed. The 16S rRNA and ITS genes were targeted in DNA extracted from maize rhizosphere soils subjected to four OF level (0 (P1/T1), 4500 (P2/T2), 9000 (P3/T3), 13500 (P4/T4) kg/ha OF applied. Compared to the control treatment, OF treatments significantly changed soil chemical properties and microbial diversity. Additionally, OF treatments also shift composition of microbial community structure and the effects on Yinchuan (P-region) were larger than Guyuan (T-region). What's more, OF treatments also significantly affected the community composition of bacteria and fungi in the two regions, among which there were significant differences in the relative abundance of three phyla, four genera of bacteria, and eight phyla and nine genera of fungi among all OF treatments. Microbial network analysis showed that the high-OF treatments (P3 and P4) reduced the number of links in the microbial network at the genus level for the 16S rRNA gene, while increased that in T-region. Redundancy analysis (RDA) revealed that organic matter (OM) and available phosphorus (AP) were the main factors driving the bacterial and fungal community structure. In conclusion, this study demonstrated that OF treatments significantly affected soil C and P as well as the bacterial and fungal community compositions in wheat rhizosphere soils, which might affect soil biogeochemical cycles.

近年来，以有机肥（OF）代替化肥是提高作物资源利用效率和农产品质量的有效途径。然而，关于 OF 对土壤​​微生物影响的知识仍然有限。通过高通量测序对银川（P）和固原（T）市两种土壤微生物群落进行研究，分析其群落结构与土壤性质的相关性。16S rRNA 和 ITS 基因在从玉米根际土壤中提取的 DNA 中靶向，这些土壤经过四个 OF 水平（0（P1/T1）、4500（P2/T2）、9000（P3/T3）、13500（P4/T4）kg/ ha OF 施用。与对照处理相比，OF 处理显着改变了土壤化学性质和微生物多样性。此外，OF处理也改变了微生物群落结构的组成，对银川（P-区）的影响大于固原（T-区）。此外，OF处理也显着影响了两个区域的细菌和真菌群落组成，其中所有OF中3门、4细菌属、8门9真菌的相对丰度存在显着差异。治疗。微生物网络分析表明，高OF处理（P3和P4）减少了16S rRNA基因属水平的微生物网络中的链接数，而增加了T区的链接数。冗余分析（RDA）显示有机质（OM）和有效磷（AP）是驱动细菌和真菌群落结构的主要因素。综上所述，