Since 30 years, bioengineering allowed to reconstruct human tissues using normal human cells. Skin has been one of the first organ to be reconstructed thanks to the development of specific cell culture media and supports favoring the culture of human skin cells such as fibroblasts, keratinocytes or melanocytes. Skin models have evolved from epidermis to complex models including a dermis. The purpose of the present study was to design a reconstructed full thickness skin suitable to perform efficacy testing of molecules and plant extracts in vitro. First, we reconstructed epidermis with normal human keratinocytes displaying the expected multi layered morphology and expressing specific epidermal proteins (e-cadherin, claudin-1, p63, Ki67, Keratin 10, filaggrin and loricrin). Then, a dermal equivalent was developed using a collagen matrix allowing the growth of fibroblasts. <b>The functionality of the dermis was demonstrated by the measurement of skin parameters such as </b>rigidity or elasticity with Ballistometer® and other parameters such as the contraction over time and the expression of dermal proteins. The combination of these two compartments (dermis and epidermis) allowed to reconstruct a full thickness model. This study model allowed to study the communication between compartments and with the establishment of a dermo epidermal junction showing the expression of specific proteins (collagen XVII, laminin, collagen IV).

中文翻译：

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新的全厚度体外皮肤模型的表征

30 年来，生物工程允许使用正常人体细胞重建人体组织。由于特定细胞培养基的发展，皮肤已成为最早被重建的器官之一，并支持培养人类皮肤细胞，如成纤维细胞、角质形成细胞或黑素细胞。皮肤模型已经从表皮演变为包括真皮在内的复杂模型。本研究的目的是设计一种重建的全层皮肤，适合在体外进行分子和植物提取物的功效测试。首先，我们用正常人角质形成细胞重建表皮，显示预期的多层形态并表达特定的表皮蛋白（e-钙粘蛋白、密蛋白-1、p63、Ki67、角蛋白 10、丝聚蛋白和loricrin）。然后，使用允许成纤维细胞生长的胶原基质开发了真皮等效物。<b>真皮的功能通过测量皮肤参数（例如</b>使用 Ballistometer® 的刚度或弹性）和其他参数（例如随时间的收缩和真皮蛋白的表达）来证明。这两个隔室（真皮和表皮）的组合允许重建全厚度模型。该研究模型允许研究隔室之间的通信以及真皮表皮连接的建立，显示特定蛋白质（胶原蛋白 XVII、层粘连蛋白、胶原蛋白 IV）的表达。Ballistometer® 和其他参数（如随时间的收缩和真皮蛋白的表达）的刚度或弹性。这两个隔室（真皮和表皮）的组合允许重建全厚度模型。该研究模型允许研究隔室之间的通信以及真皮表皮连接的建立，显示特定蛋白质（胶原蛋白 XVII、层粘连蛋白、胶原蛋白 IV）的表达。Ballistometer® 和其他参数（如随时间的收缩和真皮蛋白的表达）的刚度或弹性。这两个隔室（真皮和表皮）的组合允许重建全厚度模型。该研究模型允许研究隔室之间的通信以及真皮表皮连接的建立，显示特定蛋白质（胶原蛋白 XVII、层粘连蛋白、胶原蛋白 IV）的表达。