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Insulinoma-derived pseudo-islets for diabetes research
American Journal of Physiology-Cell Physiology ( IF 5.0 ) Pub Date : 2021-06-09 , DOI: 10.1152/ajpcell.00466.2020
Nathaniel J Hart 1 , Craig Weber 2 , Nicholas Price 1 , Alma Banuelos 1 , Madison Schultz 1 , Barry Huey 1 , Emily Harnois 2 , Cyonna Gibson 1 , Leah V Steyn 1 , Klearchos K Papas 1, 3, 4 , Ronald M Lynch 2, 3, 4
Affiliation  

The islets of Langerhans of the pancreas are the primary endocrine organ responsible for regulating whole body glucose homeostasis. The use of isolated primary islets for research development and training requires organ resection, careful digestion and isolation of the islets from non-endocrine tissue. This process is time consuming, expensive and requires substantial expertise. For these reasons, we sought to develop a more rapidly obtainable and consistent model system with characteristic islet morphology and function that could be employed to train personnel and better inform experiments prior to using isolated rodent and human islets. Immortalized β cell lines reflect several aspects of primary β cells, but cell propagation in monolayer cell culture limits their usefulness in several areas of research which depend on islet morphology and/or functional assessment. In this manuscript we describe the propagation and characterization of insulinoma pseudo-islets (IPIs) from a rat insulinoma cell line INS832/3. IPIs were generated with an average diameter of 200 μm, consistent with general islet morphology. The rates of oxygen consumption and mitochondrial oxidation-reduction changes in response to glucose and metabolic modulators were similar to isolated rat islets. In addition, the dynamic insulin secretory patterns of IPIs were similar to primary rat islets. Thus, INS832/3-derived IPIs provide a valuable and convenient model for accelerating islet and diabetes research.

中文翻译:

用于糖尿病研究的胰岛素瘤衍生的假胰岛

胰腺朗格汉斯岛是负责调节全身葡萄糖稳态的主要内分泌器官。使用分离的原代胰岛进行研究开发和培训需要器官切除、仔细消化和将胰岛与非内分泌组织隔离。这个过程耗时、昂贵并且需要大量的专业知识。出于这些原因,我们试图开发一种更快速且一致的模型系统,该模型系统具有特征性的胰岛形态和功能,可用于培训人员并在使用孤立的啮齿动物和人类胰岛之前更好地为实验提供信息。永生化 β 细胞系反映了原代 β 细胞的几个方面,但是单层细胞培养中的细胞增殖限制了它们在依赖于胰岛形态和/或功能评估的几个研究领域中的有用性。在这份手稿中,我们描述了来自大鼠胰岛素瘤细胞系 INS832/3 的胰岛素瘤假胰岛 (IPIs) 的传播和表征。IPI 的平均直径为 200 μm,与一般胰岛形态一致。响应葡萄糖和代谢调节剂的耗氧率和线粒体氧化还原变化与分离的大鼠胰岛相似。此外,IPIs 的动态胰岛素分泌模式与原代大鼠胰岛相似。因此,INS832/3 衍生的 IPI 为加速胰岛和糖尿病研究提供了一个有价值且方便的模型。在这份手稿中,我们描述了来自大鼠胰岛素瘤细胞系 INS832/3 的胰岛素瘤假胰岛 (IPIs) 的传播和表征。IPI 的平均直径为 200 μm,与一般胰岛形态一致。响应葡萄糖和代谢调节剂的耗氧率和线粒体氧化还原变化与分离的大鼠胰岛相似。此外,IPIs 的动态胰岛素分泌模式与原代大鼠胰岛相似。因此,INS832/3 衍生的 IPI 为加速胰岛和糖尿病研究提供了一个有价值且方便的模型。在这份手稿中,我们描述了来自大鼠胰岛素瘤细胞系 INS832/3 的胰岛素瘤假胰岛 (IPIs) 的传播和表征。IPI 的平均直径为 200 μm,与一般胰岛形态一致。响应葡萄糖和代谢调节剂的耗氧率和线粒体氧化还原变化与分离的大鼠胰岛相似。此外,IPIs 的动态胰岛素分泌模式与原代大鼠胰岛相似。因此,INS832/3 衍生的 IPI 为加速胰岛和糖尿病研究提供了一个有价值且方便的模型。响应葡萄糖和代谢调节剂的耗氧率和线粒体氧化还原变化与分离的大鼠胰岛相似。此外,IPIs 的动态胰岛素分泌模式与原代大鼠胰岛相似。因此,INS832/3 衍生的 IPI 为加速胰岛和糖尿病研究提供了一个有价值且方便的模型。响应葡萄糖和代谢调节剂的耗氧率和线粒体氧化还原变化与分离的大鼠胰岛相似。此外,IPIs 的动态胰岛素分泌模式与原代大鼠胰岛相似。因此,INS832/3 衍生的 IPI 为加速胰岛和糖尿病研究提供了一个有价值且方便的模型。
更新日期:2021-06-10
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